merck millipore,默克密理博,04-432,Anti-Bad (NT) Antibody, rabbit monoclonal

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merck millipore,默克密理博,04-432,Anti-Bad (NT) Antibody, rabbit monoclonal
产品名称：Anti-Bad (NT) Antibody, rabbit monoclonal
产品型号：04-432
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Bad (N-term) Antibody, Rabbit is an antibody ag More>>

merck millipore,默克密理博,04-432,Anti-Bad (NT) Antibody, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-432,Anti-Bad (NT) Antibody, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-432
品牌系列	Upstate
商名	Upstate
描述	Anti-Bad (NT) Antibody, rabbit monoclonal

产品信息
格式	Purified
演示	In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Bad (N-term) Antibody, Rabbit is an antibody against Bad (N-term) for use in IF, IP, WB, IH(P).
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to the N-terminal region of human Bad (N-term).
宿主	Rabbit
特异性	Recognizes a region near the N-terminus domain of human Bad (N-term).
同种型	IgG
品种反应性	RatHumanMouse
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_032989.1 NM_004322.2
Entrez基因汇总	The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
基因符号	BCL2L8 BAD BBC2 BBC6
UniProt编号	Q92934
UniProt汇总	FUNCTION: SwissProt: Q92934 # Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. SIZE: 168 amino acids; 18392 Da SUBUNIT: Forms heterodimers with the anti-apoptotic proteins, Bcl- X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity). SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm. TISSUE SPECIFICITY: Expressed in a wide variety of tissues. DOMAIN:SwissProt: Q92934 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro- apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation (By similarity). SIMILARITY: Belongs to the Bcl-2 family.

产品使用声明
质量保证	Routinely evaluated by immunoprecipitating
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.