merck millipore,默克密理博,2750,BrdU Cell Proliferation Kit

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Merck Millipore 默克密理愽

主要应用	检测方法
ELISA	Colorimetric

描述
产品目录编号	2750
品牌系列	Chemicon®
商名	Chemicon
描述	BrdU Cell Proliferation Kit
概述	Test Principle With CHEMICON®'s BrdU Cell Proliferation Assay Kit, mouse monoclonal antibody is used to detect the BrdU in a sample. After addition of goat anti-mouse IgG-peroxidase conjugated secondary antibody, substrate and stop solution, the amount of BrdU is determined. The higher the OD, the higher the BrdU concentration in the sample.
背景信息	Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has been a traditional method for detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting of autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well established alternative to [3H] thymidine uptake has been demonstrated by numerous researchers. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are synthesizing DNA.

产品信息
部件	50X Plate Wash Concentrate: (Part No. 2750h) One 90 mL bottle of buffered Tris with surfactant. BrdU Reagent: (Part No. 2750a) One 15 μL vial of a 500X solution of BrdU. Fixing Solution: (Part No. 2750b) One 40 mL bottle. Prediluted BrdU Detection Antibody: (Part No. 2750c) One 20 mL vial of an anti-BrdU solution. Stop Solution: (Part No. 2750d): One 25 mL bottle of 2.5N sulfuric acid solution. Goat anti-Mouse IgG, Peroxidase labeled: (Part No. 2750e) One 15 μL vial of a 2000X solution. Conjugate Diluent: (Part No. 2750f) One 25 mL bottle. Substrate: (Part No. 2750g) One 25 mL bottle, ready to use TMB.
检测方法	Colorimetric

产品信息

部件

50X Plate Wash Concentrate: (Part No. 2750h) One 90 mL bottle of buffered Tris with surfactant.
BrdU Reagent: (Part No. 2750a) One 15 μL vial of a 500X solution of BrdU.
Fixing Solution: (Part No. 2750b) One 40 mL bottle.
Prediluted BrdU Detection Antibody: (Part No. 2750c) One 20 mL vial of an anti-BrdU solution.
Stop Solution: (Part No. 2750d): One 25 mL bottle of 2.5N sulfuric acid solution.
Goat anti-Mouse IgG, Peroxidase labeled: (Part No. 2750e) One 15 μL vial of a 2000X solution.
Conjugate Diluent: (Part No. 2750f) One 25 mL bottle.
Substrate: (Part No. 2750g) One 25 mL bottle, ready to use TMB.

检测方法

Colorimetric

应用
应用	The BrdU Cell Proliferation Assay Kit is used to detect the BrdU in a sample.
主要应用	ELISA
应用说明	The CHEMICON® BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells. For Research Use Only. Not for use in diagnostic procedures.

生物信息
品种反应性	CanineMouseHumanRatPigHorseRabbitGuinea PigHamsterNon-human Primate

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain the kit at 2° to 8°C under sterile conditions for up to 6 months after date of receipt. Before first use: remove the Fixative Solution and place at room temperature for at least 4 hours prior to use. Precipitates that may occur during cold storage should go back into solution.