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检测方法 Fluorometric or Colorimetric 描述 概述 A convenient assay kit useful for screening caspase-3 inhibitors measuring the protease activity of caspase-3 and other caspase-3-like activities. Cleavage is monitored colorimetrically by measuring the increase in absorbance at 405 nm. 产品目录编号 235418 品牌系列 Calbiochem®
参考 参考 Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W. 1996. Nat. Biotechnol. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
Darmon, A.J., et al. 1995. Nature 377, 446.
Lazebnik, Y.A., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9042.
Nicholson, D.W., et al. 1995. Nature 376, 37.
Tewari, M., et al. 1995. Cell 81, 801.
Fernandes-Alnemri, T., et al. 1994. J. Biol. Chem. 269, 30761.产品信息 检测方法 Fluorometric or Colorimetric 形式 96 Tests 格式 96-well plate 试剂盒包含 Human Recombinant Caspase-3 (Cat. No. 235417), a DEVD-pNA Colorimetric Substrate, DEVD-AMC Fluorometric Substrate, Calibration Standard I (p-Nitroaniline), Calibration Standard II (AMC), an Inhibitor, Assay Buffer, ½-volume 96-Well Plate, and a user protocol. 生物信息 化验时间 2 h 样品类型 Recombinant or purified caspase-3 安全信息 R相 R: 23/24/25-63-36/37/38-33
Toxic by inhalation, in contact with skin and if swallowed.
Possible risk of harm to the unborn child.
Irritating to eyes, respiratory system and skin.
Danger of cumulative effects.S相 S: 36/37/39-45-26-23
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Do not breathe fumes.储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 ≤ -70°C
存储条件 Upon arrival of kit store entire kit Contents (except 1/2 volume well plate, store at room temperature) at -70°C.
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Human Recombinant Caspase-3 (Cat. No. 235417), a DEVD-pNA Colorimetric Substrate, DEVD-AMC Fluorometric Substrate, Calibration Standard I (p-Nitroaniline), Calibration Standard II (AMC), an Inhibitor, Assay Buffer, ½-volume 96-Well Plate, and a user protocol.
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