• merck millipore,默克密理博,219350,Anti-β-Catenin, Dephospho Mouse mAb (8E4)
  • merck millipore,默克密理博,219350,Anti-β-Catenin, Dephospho Mouse mAb (8E4)

    产品名称:Anti-β-Catenin, Dephospho Mouse mAb (8E4)
    产品型号:219350
    This Anti-β-Catenin, Dephospho Mouse mAb (8E4) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of β-Catenin, Dephospho.

    merck millipore,默克密理博,219350,Anti-β-Catenin, Dephospho Mouse mAb (8E4)

  • 产品介绍
  • merck millipore,默克密理博,219350,Anti-β-Catenin, Dephospho Mouse mAb (8E4)

    重要规格表

    宿主
    M
    描述
    概述 Recognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
    产品目录编号 219350
    品牌系列 Calbiochem®
    应用数据
    Detection of human dephospho-β-catenin by immunoblotting. Samples: Whole cell lysates of serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-β-Catenin, Dephospho Mouse mAb (8E4) (Cat. No. 219350) (0.5 µg/ml). Detection: chemiluminescence.
    产品信息
    形式 Lyophilized
    配方 100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
    正控制 LiCl-treated SW480 cells (included)
    应用
    主要应用 Enzyme-Linked Immunosorbent Assay
    Immunoblotting (Western Blotting)
    Immunoprecipitation
    生物信息
    免疫原 a synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
    免疫原品种 Mouse
    克隆 8E4
    宿主 Mouse
    同种型 IgG₁
    品种 human, mouse, rat
    储存和货运信息
    货运代码 Ambient Temperature Only
    毒性 Standard Handling
    储存 -20°C
    无需冷冻 No
    特殊指南 Reconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.

    merck millipore,默克密理博,219350,Anti-β-Catenin, Dephospho Mouse mAb (8E4)

上一件merck millipore产品:merck millipore,默克密理博,07-061,Anti-MST1/Krs2 Antibody
下一件merck millipore产品:merck millipore,默克密理博,SF-1747,IL7R; Human, Cyanine 5 RNA Detection Probe,SmartFlare



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