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merck millipore,默克密理博,219350,Anti-β-Catenin, Dephospho Mouse mAb (8E4)
产品名称:Anti-β-Catenin, Dephospho Mouse mAb (8E4)
产品型号:219350
This Anti-β-Catenin, Dephospho Mouse mAb (8E4) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of β-Catenin, Dephospho.
merck millipore,默克密理博,219350,Anti-β-Catenin, Dephospho Mouse mAb (8E4)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells. 产品目录编号 219350 品牌系列 Calbiochem®
应用数据 
Detection of human dephospho-β-catenin by immunoblotting. Samples: Whole cell lysates of serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-β-Catenin, Dephospho Mouse mAb (8E4) (Cat. No. 219350) (0.5 µg/ml). Detection: chemiluminescence.产品信息 形式 Lyophilized 配方 100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate. 正控制 LiCl-treated SW480 cells (included) 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
生物信息 免疫原 a synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH 免疫原品种 Mouse 克隆 8E4 宿主 Mouse 同种型 IgG₁ 品种 human, mouse, rat 储存和货运信息 货运代码 Ambient Temperature Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Reconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
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