merck millipore,默克密理博,17-701,EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

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merck millipore,默克密理博,17-701,EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
产品名称：EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
产品型号：17-701
RNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads. Control primers More>>

merck millipore,默克密理博,17-701,EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

产品介绍

merck millipore,默克密理博,17-701,EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit

重要规格表

主要应用
RIP

描述
产品目录编号	17-701
品牌系列	Upstate
商名	Upstate EZ-Magna RIP
描述	EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit
概述	RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq). Features & Benefits: -Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes -RNAse inhibitors and RNAse-free reagents -Positive and negative controls
Alternate Names	Magnetic RIP kit
背景信息	Gene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.

产品信息
部件	Control Primers Magnetic Beads Protein A/G RIP Wash Buffer RIP Lysis Buffer 0.5 M EDTA 10% SDS Salt Solution I Salt Solution II Precipitate Enhancer Normal Mouse IgG Rabbit IgG Purified Protease Inhibitor Cocktail 200X RNase Inhibitor Proteinase K (10 mg/mL) Nuclease free water Positive Control Antibody (Anti-SNRNP70)
演示	Three boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions. Includes positive control antibody and control primers.

应用
应用	RNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads. Control primers included.
主要应用	RNA Binding Protein Immunoprecipitation (RIP)

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.