merck millipore,默克密理愽
  • merck millipore,默克密理博,17-245,Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit

  • merck millipore,默克密理博,17-245,Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit

    产品名称：Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit
    产品型号：17-245
    For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3.

    merck millipore,默克密理博,17-245,Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit

  • 产品介绍
  • merck millipore,默克密理博,17-245,Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit

    重要规格表

    主要应用
    IP
    描述
    产品目录编号 17-245
    品牌系列 Upstate
    商名
    • Upstate
    描述 Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit
    概述 For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher using quantitative PCR or Southern slot-blot analysis with promotor specific primers or probe.
    产品信息
    部件
    • All necessary buffers.
    • Anti-acetyl-Histone H3 (Cat.# 06-599)
    • Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)
    应用
    应用 For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3.
    主要应用
    • Immunoprecipitation
    生物信息
    Entrez基因编号
    • NM_003493.2
    Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
    基因符号
    • MGC126886
    • HIST3H3
    • H3FT
    • H3/t
    • H3t
    • MGC126888
    • H3/g
    • H3.4
    • H3T
    修改
    • Acetylation
    UniProt编号
    • Q16695
    UniProt汇总 FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    SIZE: 136 amino acids; 15508 Da
    SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
    SUBCELLULAR LOCATION: Nucleus.
    TISSUE SPECIFICITY: Expressed in testicular cells.
    DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
    PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
    SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 1 kit
    包装 Kit capacity: 22 assays

    merck millipore,默克密理博,17-245,Acetyl-Histone H3 Immunoprecipitation (ChIP) Assay Kit

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