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merck millipore,默克密理博,17-10520,Magna Nuclear RIP™ (Cross-Linked)Nuclear RNA-Binding Protein Immunoprecipitation Kit
产品名称：Magna Nuclear RIP™ (Cross-Linked)Nuclear RNA-Binding Protein Immunoprecipitation Kit
产品型号：17-10520
Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs.
merck millipore,默克密理博,17-10520,Magna Nuclear RIP™ (Cross-Linked)Nuclear RNA-Binding Protein Immunoprecipitation Kit
- 产品介绍
- merck millipore,默克密理博,17-10520,Magna Nuclear RIP™ (Cross-Linked)Nuclear RNA-Binding Protein Immunoprecipitation Kit
重要规格表
描述 产品目录编号 17-10520 商名 - Magna Nuclear RIP
描述 Magna Nuclear RIP™ (Cross-Linked)
Nuclear RNA-Binding Protein Immunoprecipitation Kit概述 Features and Advantages - Generates cross-linked chromatin to allow analysis of a variety of chromatin-associated RNAs
- Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 5,000 cells
- Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios
- Suitable for analysis by RT-qPCR or RIP-seq
- Complete set of reagents and detailed protocol to enable first-time success
Magna Nuclear RIP™ Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq).
Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Cross-linked can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. In contrast native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. For less well understood proteins and protein complexes often both approaches are used.
The kit described here is for the cross-linked approach. If a native approach is of interest please visit the product page for the Magna Nuclear RIP (Native) Kit, catalogue # 17-10522 or the EZ-Magna Nuclear RIP (Native) Kit, catalogue # 17-10523.产品信息 部件 - Proteinase K
- 10X Glycine
- 10X PBS
- Nuclei Isolation Buffer
- RIP Cross-Linked Lysis Buffer
- Protein A/G Magnetic Beads
- Nuclear RIP Dilution Buffer
- Low Salt Wash Buffer
- High Salt Wash Buffer
- LiCl Wash Buffer
- TE Buffer
- RIP Elution Buffer
- 10% SDS
- 0.5 M EDTA
- DNase I (RNase Free)
- DNase I Supplement
- DNase I Reaction Buffer
- Protease Inhibitor Cocktail III, Animal Free
- RNAse Inhibitor
演示 Two boxes containing key reagents for generation of cross linked nuclear lysates and performance of 24 individual RNA-binding protein immunoprecipitation (RIP) reactions. 应用 应用 Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs. 主要应用 - Protein Interaction Assays
- RNA Binding Protein Immunoprecipitation (RIP)
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Upon receipt, store components at the temperatures indicated on the labels.
Kit components are stable for 6 months from date of shipment when stored as directed.包装信息 数量 24 assays 包装 Kit capacity: 24 RNA-binding protein immunoprecipitation assays using a cross-linked nuclear lysate
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