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merck millipore,默克密理博,17-10461,EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit
产品名称：EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit
产品型号：17-10461
Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads. More>>
merck millipore,默克密理博,17-10461,EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit
- 产品介绍
重要规格表
描述 产品目录编号 17-10461 品牌系列 Upstate
商名 - Upstate
- EZ-Magna ChIP
描述 EZ-Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit 概述 The EZ-Magna HiSens ChIP kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating the need to purchase different kits for different antibody isotypes. The SCW Buffer is unique to the Magna ChIP HiSens kit and enables the use of a single buffer for multiple steps of the ChIP process (sonication, chromatin immunoprecipitation, and wash). The ChIP elution buffer provided with the HiSens kit has been formulated to allow analysis of enrichment by qPCR without additional clean-up steps for more rapid results.
Features & Benefits- Robust ChIP from as few as 10,000 to as many as 1,000,000 cells
- Protocols and reagents for generation of chromatin from range of input sample type
- Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies
- Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment
- Single buffer system for sonication, chromatin IP, and wash
- Perform analysis of enrichment without additional purification after cross link reversal.
- Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray
- Includes positive and negative control antibodies and assay against known positive locus
Alternate Names - low-input chromatin immunoprecipitation kit
- Low-input ChIP kit
背景信息 Chromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes. 产品信息 部件 - Control PCR primers
- 10X Glycine
- Nuclei Isolation Buffer
- 10X PBS
- SCW Buffer (Sonication/ChIP/Wash)
- Magna ChIP Protein A/G Magnetic Beads
- Low Stringency IP Wash Buffer
- ChIP Elution Buffer
- Protease Inhibitor Cocktail
- Proteinase K Solution
- Anti-Trimethyl-Histone H3 (Lys4)
- Normal Rabbit IgG
演示 Two boxes containing all necessary reagents to perform up to 24 chromatin immunoprecipitation reactions. Supplied buffers are sufficient to generate 12 individual chromatin preparation reactions using 15 cm plates of cultured cells or tissue samples. 应用 应用 Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads. Control primers included. 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed. 包装信息 数量 24 assays 包装 Kit capacity: 24 chromatin immunoprecipitation assays
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