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merck millipore,默克密理博,16-658,Anti-acetyl-Histone H3 Antibody, Agarose Conjugate
产品名称：Anti-acetyl-Histone H3 Antibody, Agarose Conjugate
产品型号：16-658
Anti-acetyl-Histone H3 Antibody, Agarose Conjugate is a Rabbit Polyclonal Antibody for the detection of acetyl-Histone H3 & has been validated in IP.
merck millipore,默克密理博,16-658,Anti-acetyl-Histone H3 Antibody, Agarose Conjugate
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 T, H, M IP Rb Gel Immobilized Polyclonal Antibody 描述 产品目录编号 16-658 描述 Anti-acetyl-Histone H3 Antibody, Agarose Conjugate Alternate Names - H3 histone, family 3A
- H3 histone
背景信息 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysines in histone H3 is associated with genes that are being actively transcribed into RNA. 产品信息 格式 Gel Immobilized 控制 - TSA-treated or sodium butyrate-treated HeLa or NIH/3T3 cells.
演示 Purified rabbit IgG (anti-acetyl histone H3, Cat. #06-599) conjugated to protein A agarose. Supplied as a 50% slurry (100 μL packed beads) in PBS with 0.05% sodium azide. 应用 应用 Anti-acetyl-Histone H3 Antibody, Agarose Conjugate is a Rabbit Polyclonal Antibody for the detection of acetyl-Histone H3 & has been validated in IP. 主要应用 - Immunoprecipitation
应用说明 Immunoprecipitation:
Acetylated histone H3 was precipitated from sodium butyrate-treated HeLa acid extracts.
生物信息 免疫原品种 KLH-conjugated peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 (ARTKQTAR[K*]STGG[K*]APRKQLC) where K* is acetylated. 表位 a.a. 1-20 宿主 Rabbit 特异性 Acetylated histone H3. 同种型 IgG 品种反应性 TetrahymenaHumanMouse Species Reactivity Note Due to immunogen conservation, wide species reactivity is expected. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003493
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
基因符号 - OTTHUMP00000035621
- H3.3A
- H3.3B
- H3F3
- MGC87782
- MGC87783
- OTTHUMP00000035618
- OTTHUMP00000035619
修改 - Acetylation
纯化方法 Antibody was purified by protein A chromatography prior to conjugation. UniProt编号 - P84243
UniProt汇总 FUNCTION: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Ref.14 Ref.18 Ref.22
SUBUNIT STRUCTURE:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Ref.14
SUBCELLULAR LOCATION: Nucleus.
DEVELOPMENTAL STAGE: Expressed throughout the cell cycle independently of DNA synthesis.
PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18.
Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription.
Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression.
Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5, Lys-37 and Lys-80. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28, which are linked to gene repression, are underrepresented. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin.
Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase, probably DAPK3. Phosphorylation at 'Ser-11' by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase chromosomes. Ref.9 Ref.10 Ref.12 Ref.13 Ref.19 Ref.20 Ref.21 Ref.29
Ubiquitinated By similarity.
SIMILARITY: Belongs to the histone H3 family.
SEQUENCE CAUTION:The sequence CAH73371.1 differs from that shown. Reason: Erroneous gene model prediction.
产品使用声明 质量保证 Immunoprecipitation:
Acetylated histone H3 was precipitated from sodium butyrate-treated HeLa acid extracts.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 200 µL
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