merck millipore,默克密理博,15-115,AMPK Pathway Explorer Antibody MiniPack

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merck millipore,默克密理博,15-115,AMPK Pathway Explorer Antibody MiniPack
产品名称：AMPK Pathway Explorer Antibody MiniPack
产品型号：15-115
This Antibody pack contains Anti-AMPK α1 Antibody, Anti-AMPKα2 Antibody, Anti-phospho-AMPKα Antibody(Thr172).

merck millipore,默克密理博,15-115,AMPK Pathway Explorer Antibody MiniPack

产品介绍

merck millipore,默克密理博,15-115,AMPK Pathway Explorer Antibody MiniPack

描述
产品目录编号	15-115
品牌系列	Upstate
商名	Upstate
描述	AMPK Pathway Explorer Antibody MiniPack
Alternate Names	AMP-Activated Protein Kinase
背景信息	Pathway Explorer Antibody MiniPack: Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591). AMPK: AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity. *See full size versions for corresponding references.

产品信息
部件	07-681SP Anti-phospho-AMPKα (Thr172); 60 µg 07-350SP Anti-AMPK α1; 60 µg 07-363SP Anti-AMPKα2; 60 µg
格式	Purified
控制	For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock. For 07-681SP use HEK293 cells or rat liver AMPK. For 07-363SP use Rat liver preparation.
演示	3 individual tubes containing either Anti-AMPK α1; Anti-AMPKα2; or Anti-phospho-AMPKα (Thr172)
性质	Each vial is 30% the size of the parent catalog number

应用
应用	This Antibody pack contains Anti-AMPK α1 Antibody, Anti-AMPKα2 Antibody, Anti-phospho-AMPKα Antibody(Thr172).
主要应用	Western Blotting

生物信息
免疫原品种	Varies. See individual Data Sheets
表位	07-350SP and 07-363SP: Internal
浓缩	1 mg/mL
宿主	Rabbit
特异性	07-681SP: AMPKα phosphorylated on Thr172. 07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2). 07-350SP: AMPKα1
品种反应性	RatHumanMouse
Species Reactivity Note	07-681SP:Human and rat. Expected to cross-react with mouse based on 100% sequence homology. 07-363SP: Human and rat. 07-350SP: Human, rat, and mouse.
抗体类型	Polyclonal Antibody
交叉反应性	Varies. See individual Data Sheets
Entrez基因编号	NP_006243 NP_006242
Entrez基因汇总	AMPK: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
基因符号	AMPK
修改	Phosphorylation
纯化方法	Protein A purfied
UniProt编号	Q13131
UniProt汇总	FUNCTION: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. Ref.8 Ref.9 Catalytic activity ATP + a protein = ADP + a phosphoprotein. Cofactor Magnesium. ENZYME REGULATION: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. Ref.8 Ref.9 SUBUNIT STRUCTURE: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2. Ref.14 Ref.15 SEQUENCE SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain. SEQUENCE CAUTION: The sequence AAH48980.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened

产品使用声明
质量保证	07-681SP:Routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK. Western Blot Analysis: 0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase. 07-363SP: Routinely evaluated by immunoblot in a rat liver preparation. Western Blot Analysis: 1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation. 07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells. Western Blot Analysis: 0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

07-681SP:Routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.

07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.

Western Blot Analysis:
1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation.

07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.