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merck millipore,默克密理博,HTS163M,ChemiSCREEN™ Membrane Preparation Recombinant Human GLP-1
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human GLP-1
产品型号：HTS163M
Human GLP-1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS163M,ChemiSCREEN™ Membrane Preparation Recombinant Human GLP-1
- 产品介绍
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-9 GLP-1 描述 产品目录编号 HTS163M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human GLP-1 概述 Human GLP-1 背景信息 Glucagon-like peptide-I (GLP-1), a member of the glucagon-secretin peptide family, is secreted from L-cells of the small intestine and binds to a class B (class 2) G protein-coupled receptor (Mayo et al. 2003). The GLP-1 receptor is expressed in pancreatic beta cells and upon binding to GLP-1, it couples to Gs to increase cAMP levels and insulin secretion (Drucker et al. 1987). In addition, GLP-1 has been shown to delay gastric emptying and regulate appetite. Therefore, the GLP-1 receptor represents an important therapeutic target for type II diabetes, and a degradation-resistant analog of GLP-1, exanatide, is used clinically in combination with other glucose-lowering drugs to control type II diabetes (D’Alessio et al. 2004). Millipore's GLP-1 receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists at the GLP-1 receptor. The membrane preparations exhibit a Kd of 0.27 nM for [125I]-GLP-1. With 10 μg/well GLP-1 Receptor Membrane Prep and 0.5 nM [125I]-GLP-1, a greater than 9-fold signal-to-background ratio was obtained. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 9-fold signal:background with 125I-labeled GLP-1 at 0.5 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human GLP-1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_002062.2
基因符号 - GLP-1-R
- GLP1R
- MGC138331
- GLP-1R
GPCR类别 B 宿主细胞 Chem-9 蛋白靶 GLP-1 靶子系列 Glucagon UniProt编号 - P43220
UniProt汇总 FUNCTION: SwissProt: P43220 # This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 463 amino acids; 53060 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P43220 ## Belongs to the G-protein coupled receptor 2 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I] GLP-1(7-36) (Perkin Elmer # NEX308)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.产品使用声明 质量保证 SPECIFICATIONS:
Signal:background and specific binding values obtained in a competition binding assay with varying amounts of GLP-1 membrane prep:
20 µg/well 10 µg/well Signal:Background 12.0 14.9 Specific Binding (cpm) 31366 27411
1 unit = 10 µg membrane preparation
Bmax: 3.96 pmol/mg
Kd: 0.27 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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