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描述 产品目录编号 14-440M Replaces 14-114 品牌系列 Upstate
商名 - Upstate
描述 PKA Protein, Recombinant, 250 µg 概述 Recombinant human full length PKA, catalytic subunit type alpha, untagged 应用 应用 Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 来源 Expressed in E.coli BL21 (DE3) pLysS cells 品种 Human 特异活性 For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. Entrez基因编号 - NM_207518.1
- NM_002730.3
Entrez基因汇总 cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. 基因符号 - MGC48865
- PRKACA
- MGC102831
- PKACA
蛋白靶 PKA 纯化方法 Phosphocellulose P11 chromatography 靶子系列 AGC UniProt编号 - P17612
UniProt汇总 FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
SIZE: 351 amino acids; 40590 Da
SUBUNIT: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
TISSUE SPECIFICITY: Isoform 2 is sperm specific.
PTM: Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity).
SIMILARITY: SwissProt: P17612 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.产品使用声明 质量保证 routinely evaluated by phosphorylation of Kemptide substrate in vitro 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -70°C 包装信息 数量 250 µg 包装 Also available in 10 μg size (cat#14-440) and in bulk (cat# 14-440-K).
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