• merck millipore,默克密理博,14-1064,Human Recombinant Histone H3.3 K27M
  • merck millipore,默克密理博,14-1064,Human Recombinant Histone H3.3 K27M

    产品名称:Human Recombinant Histone H3.3 K27M
    产品型号:14-1064
    Human recombinant histone H3.3 K27M mutant (Lysine to Methionine) expressed in E Coli & purified by Fast Protein Liquid Chromatography (FPLC) & verified mass spectrometry.

    merck millipore,默克密理博,14-1064,Human Recombinant Histone H3.3 K27M

  • 产品介绍
  • merck millipore,默克密理博,14-1064,Human Recombinant Histone H3.3 K27M

    重要规格表

    主要应用
    ACT
    描述
    产品目录编号 14-1064
    商名
    • Upstate
    描述 Human Recombinant Histone H3.3 K27M
    概述 Human recombinant histone H3.3 K27M (lysine 27 to methionine mutant) expressed in E Coli. and purified by Fast Protein Liquid Chromatography (FPLC).
    背景信息 The core histones, H2A, H2B, H3, and H4, form a central octameric structure that binds approximately 147 bp of DNA to form a nucleosome. The nucleosome is the first order of DNA compaction in the nucleus, and is the basic unit of chromatin. The structure of chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of H2A, H2B, H3, and H4. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin, and essential tools such as histone proteins and appropriate enzymes, to assemble chromatin in vitro. Mutations of lysine 27 (K27) to methionine (M), have been associated with the development of pediatric glioblastoma (see Lewis, P. W. et al. Inhibition of PRC2 activity by a gain-of-function H3 mutation found in pediatric glioblastoma. Science 28 Mar 2013).
    产品信息
    演示 In a buffer containing 20mM sodium phosphate pH 7.0, 1mM EDTA, 0.3 M NaCl, 0.5mM PMSF and 1mM DTT.
    应用
    应用 Human recombinant histone H3.3 K27M mutant (Lysine to Methionine) expressed in E Coli & purified by Fast Protein Liquid Chromatography (FPLC) & verified mass spectrometry.
    主要应用
    • Activity Assay
    应用说明 Suitable for in vitro chromatin assembly and enzyme activity assays
    生物信息
    浓缩 1.0 µg/µL
    宿主 E. coli
    纯度 Greater than 98% purity by SDS PAGE
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable at -80ºC for 1 year from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,14-1064,Human Recombinant Histone H3.3 K27M

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