merck millipore,默克密理愽
  • merck millipore,默克密理博,MABE350,Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

  • merck millipore,默克密理博,MABE350,Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

    产品名称：Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12
    产品型号：MABE350
    Detect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP.

    merck millipore,默克密理博,MABE350,Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

  • 产品介绍
  • merck millipore,默克密理博,MABE350,Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HChIP, WBRPurifiedMonoclonal Antibody
    描述
    产品目录编号 MABE350
    描述 Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12
    Alternate Names
    • RNA-directed RNA polymerase II subunit RPB1
    • DNA-directed RNA polymerase II subunit RPB1
    • RNA polymerase II subunit B1
    • DNA-directed RNA polymerase II subunit A
    • DNA-directed RNA polymerase III largest subunit
    背景信息 RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
    产品信息
    格式 Purified
    控制
    • Untreated and lambda phosphatase treated HeLa cell lysates
    演示 Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Detect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP.
    主要应用
    • Chromatin Immunoprecipitation (ChIP)
    • Western Blotting
    应用说明 Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).

    Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).
    生物信息
    免疫原品种 Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Tyr1.
    克隆 3D12
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rat
    同种型 IgG1κ
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with Human and Yeast.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_000928
    基因符号
    • POLR2
    • POLR2A
    纯化方法 Protein G purified
    UniProt编号
    • P24928
    产品使用声明
    质量保证 Evaluated by Western Blot Analysis in untreated and lambda phosphatase treated HeLa cell lysates.

    Western Blot Analysis: 2 µg/mL of this antibody detected phospho-RNAPII (Tyr1) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MABE350,Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

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