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描述 产品目录编号 12-457 品牌系列 Upstate
商名 - Upstate
描述 MYPT1 (654-880) 概述 ROK substrate protein corresponding to amino acids 654-880 of human MYPT1 with an N-terminal GST tag. MW = 60 kDa. 产品信息 演示 100mM Tris-HCl pH 8.0, 200mM NaCl, 10mM glutathione and 5μg/ml leupeptin. Frozen solution. 应用 主要应用 - Kinase Assay
生物信息 纯度 ≥70% as judged by SDS PAGE and Coomassie staining. 来源 expressed in E. coli Entrez基因编号 - NM_002480.1
Entrez基因汇总 Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. 基因符号 - MBS
- PPP1R12A
- MYPT1
- MGC133042
UniProt编号 - O14974
UniProt汇总 FUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.
SIZE: 1030 amino acids; 115281 Da
SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
PTM: Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: O14974 ## Contains 6 ANK repeats.产品使用声明 质量保证 Routinely evaluated as a substrate in a Protein Kinase Assay. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery, centrifuge the vial prior to removing the cap. 包装信息 数量 100 µg
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