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merck millipore,默克密理博,09-812,Anti-JMJD6 Antibody
产品名称：Anti-JMJD6 Antibody
产品型号：09-812
Anti-JMJD6 Antibody is a rabbit polyclonal antibody for detection of JMJD6 also known as jumonji domain containing 6, Protein PTDSR, Phosphatidylserine receptor, Lysyl-hydroxylase JMJD6 & has been val More>>
merck millipore,默克密理博,09-812,Anti-JMJD6 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-812 描述 Anti-JMJD6 Antibody Alternate Names - Histone arginine demethylase JMJD63
- JMJD
- jumonji domain containing 6
- Protein PTDSR
- Phosphatidylserine receptor
- Lysyl-hydroxylase JMJD6
- Jumonji domain-containing protein 6
- JmjC domain-containing protein 6
- Peptide-lysine 5-dioxygenase JMJD6
背景信息 JmjC domain-containing protein 6 (JMJD6) is a dioxygenase belonging to group of proteins known as the Jmjc-domain containing proteins. This family of proteins is a group of redox enzymes specifically involved in catalyzing oxidation reactions, and are characterized by the presence of a highly conserved jumonji C (Jmjc)-domain. JMJD6 can function both as a lysyl-hydroxylase and a histone arginine demethylase. As a lysyl-hydroxylase, JMJD6 catalyzes 5-hydroxylation of target protein lysine residues. As an arginine demethylase, JMJD6 has a critical part to play in histone code by demethylating of histone H3 and histone H4. It has also been shown to mediate U2AF2/U2AF65 5-hydrozylation, thereby functioning as a regulator for RNA splicing through affecting U2AF2/U2AF65 pre-mRNA activity. JMJD6 is a critical component of embryogenesis for the differentiation of various organs, and may act as a regulator for hematopoietic regulation and macrophage cytokine response. High expression of JMJD6 has been observed in heart, kidney and skeletal muscle. Medium to low expression has been seen in brain, liver, lung, ovary, pancreas, placenta, prostate, testis, thymus, and spleen. 产品信息 格式 Affinity Purified 控制 - HeLa nuclear extract
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-JMJD6 Antibody is a rabbit polyclonal antibody for detection of JMJD6 also known as jumonji domain containing 6, Protein PTDSR, Phosphatidylserine receptor, Lysyl-hydroxylase JMJD6 & has been validated in WB, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: 1:500 dilution of this antibody has been shown to detect JMJD6 in HeLa and A431 cells. 生物信息 免疫原品种 Linear peptide corresponding to human JMJD6. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes JMJD6. 品种反应性 Human Species Reactivity Note Demonstrated to react with human. Predicted to react with Xenopus based on 100% sequence homology.
抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001074930
Entrez基因汇总 This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - PSR
- JMJD6
- PTDSR1
- PTDSR
纯化方法 Affinity purified UniProt编号 - Q6NYC1
UniProt汇总 FUNCTION: Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a central role in histone code. Also acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. Required for differentiation of multiple organs during embryogenesis. Probably acts as a key regulator of hematopoietic differentiation. Seems to be necessary for the regulation of macrophage cytokine responses.
COFACTOR: Binds 1 Fe2+ ion per subunit.
SUBUNIT STRUCTURE: Interacts with LUC7L2, LUC7L3 and U2AF2/U2AF65.
SUBCLELLULAR LOCATION: Nucleus
TISSUE SPECIFICTY: Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells.
INDUCTION: Up-regulated upon cytokine treatment, but not upon TNF-alpha treatment.
DOMAIN: The nuclear localization signal motifs are necessary and sufficient to target it into the nucleus.
SEUQENCE SIMILARITIES: Belongs to the JMJD6 family.
Contains 1 JmjC domain.
CAUTION: Was initially thought to constitute the phosphatidylserine receptor, a receptor that mediates recognition of phosphatidylserine, a specific marker only present at the surface of apoptotic cells. Phosphatidylserine receptor probably participates in apoptotic cell phagocytosis. This protein was identified using phage display expressing mAb 217, an antibody that specifically recognizes phosphatidylserine receptor. However, its nuclear localization and the fact that mAb 217 antibody still recognizes the phosphatidylserine receptor in mice lacking JMJD6, strongly suggest that it does not constitute the receptor for phosphatidylserine and is not involved in apoptotic cell removal.
SEQUENCE CAUTION: The sequence AAH47003.1 differs from that shown. Reason: Erroneous initiation.
The sequence BAA25511.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 1 µg/mL of this antibody detected JMJD6 on 10 µg of HeLa nuclear extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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