merck millipore,默克密理愽
  • merck millipore,默克密理博,09-272,Anti-phospho-p38α (Thr180/Tyr182) Antibody

  • merck millipore,默克密理博,09-272,Anti-phospho-p38α (Thr180/Tyr182) Antibody

    产品名称：Anti-phospho-p38α (Thr180/Tyr182) Antibody
    产品型号：09-272
    This Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).

    merck millipore,默克密理博,09-272,Anti-phospho-p38α (Thr180/Tyr182) Antibody

  • 产品介绍
  • merck millipore,默克密理博,09-272,Anti-phospho-p38α (Thr180/Tyr182) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 09-272
    Replaces AB3828
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-phospho-p38α (Thr180/Tyr182) Antibody
    Alternate Names
    • SAPK2a, MAPK14, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, p38ALPHA, mitogen-activated protein kinase 14
    • SAPK2a
    • MAPK14
    • CSBP1
    • CSBP2
    • CSPB1
    • PRKM14
    • PRKM15
    • p38ALPHA
    产品信息
    格式 Affinity Purified
    控制
    • Anisomycin treated HeLa cells
    演示 Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
    应用
    应用 This Anti-phospho-p38α (Thr180/Tyr182) Antibody is validated for use in IP, WB for the detection of phospho-p38α (Thr180/Tyr182).
    主要应用
    • Western Blotting
    应用说明 Immunoblot Analysis: A 1:1,000 dilution of the lot detected p38α dually phosphorylated on residues Thr180 and Tyr182 in lysate from HeLa cells stimulated with anisomycin.

    Immunfluorescence: A 1:100 dilution of this antibody detected p38α phosphorylated on both residues, Thr180 and Tyr182, in fixed HeLa cells stimulated with anisomycin.

    Immunoprecipitation (IP): 2-5 μL (5-10 μL/mL) of this antibody immunoprecipitated both active p38α from anisomycin treated HeLa whole cell extract and human recombinant GST-p38α, separately, in RIPA buffer.

    Immunoprecipitation Kinase Reaction (IPK): 2-5 μL of this lot immunoprecipitated phosphorylated p38α from both anisomycin treated HeLa cells as well as recombinant p38α, separately, and then placed into an in vitro Kinase reaction with recombinant GST-ATF-2 where it phosphorylated ATF-2 on Thr69/71.
    生物信息
    免疫原品种 Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-p38α (Thr180/Tyr182).
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes p38α dual phosphorylation of Thr180 and Tyr182.
    同种型 IgG
    品种反应性 RatHumanMouse
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_139014.1
    • NM_001315.1
    • NM_139012.1
    • NM_139013.1
    Entrez基因汇总 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
    基因符号
    • CSBP2
    • MAPK14
    • PRKM14
    • RK
    • SAPK2A
    • CSBP1
    • p38
    • CSPB1
    • CSBP
    • PRKM15
    • Mxi2
    • p38ALPHA
    • MXI2
    • EXIP
    修改
    • Phosphorylation
    纯化方法 Immunoaffinity purified
    UniProt编号
    • Q16539
    UniProt汇总 FUNCTION: SwissProt: Q16539 # Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
    COFACTOR: Magnesium.
    SIZE: 360 amino acids; 41293 Da
    SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (By similarity). Interacts with NP60.
    SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
    TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR.
    SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
    产品使用声明
    质量保证 Routinely evaluated by immunoblot.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,09-272,Anti-phospho-p38α (Thr180/Tyr182) Antibody

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