merck millipore,默克密理博,08-142,SUMO1 Protein

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merck millipore,默克密理博,08-142,SUMO1 Protein
产品名称：SUMO1 Protein
产品型号：08-142

merck millipore,默克密理博,08-142,SUMO1 Protein

产品介绍

merck millipore,默克密理博,08-142,SUMO1 Protein

描述
产品目录编号	08-142
品牌系列	Upstate
商名	Upstate
描述	SUMO1 Protein
概述	The small ubiquitin-related modifier SUMO-1 belongs to the growing family of ubiquitin-related proteins involved in post translational protein modification1. It is present in all eukaryotic kingdoms and is highly conserved from yeast to humans. SUMO-1 does not appear to target proteins for degradation but seems to be involved in the modulation of protein-protein interactions. SUMO-1 and the close homologues SUMO-2 and SUMO-3 with some 50% homology between SUMO-1 and SUMO-2/3.

产品信息
演示	100 µg of concentration 0.5 mg/mL in 20mM TRIS-HCl, pH7.5, 1mM DTT.

应用
应用说明	Mature SUMO-1 suitable for in vitro SUMOylation using the SUMO Activating Enzyme Complex (Cat. no. 14-858) and SUMO conjugating enzyme UbcH9 (Cat. no. 14-815). In The presence of these enzymes with ATP, Mg2+, and DTT, mature SUMO-1 may be conjugated to soluble proteins. The protein is N-terminally His6-tagged within a leader sequence. Typical in vitro concentration for non-rate limiting conjugate formation is 200μM to 1mM depending upon conditions.

应用

应用说明

Mature SUMO-1 suitable for in vitro SUMOylation using the SUMO Activating Enzyme Complex (Cat. no. 14-858) and SUMO conjugating enzyme UbcH9 (Cat. no. 14-815). In The presence of these enzymes with ATP, Mg2+, and DTT, mature SUMO-1 may be conjugated to soluble proteins. The protein is N-terminally His6-tagged within a leader sequence. Typical in vitro concentration for non-rate limiting conjugate formation is 200μM to 1mM depending upon conditions.

生物信息
浓缩	0.5 mg/mL
纯度	≥95%
品种	Human
Entrez基因编号	NM_003352.4 NM_001005781.1 NM_001005782.1
Entrez基因汇总	This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.
基因符号	SMT3C SUMO1 sentrin Sentrin SMT3H3 OFC10 UBL1 DAP-1 SENP2 SMT3 PIC1 GMP1 SUMO-1
UniProt编号	P63165
UniProt汇总	FUNCTION: SwissProt: P63165 # Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1- SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. SIZE: 101 amino acids; 11557 Da SUBUNIT: Interacts with SAE2, UBE2I, RANBP2, PIAS1 and PIAS2. Interacts with PARK2. Covalently attached to a number of proteins such as PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52. SUBCELLULAR LOCATION: Nucleus membrane. Nucleus speckle. Cytoplasm. PTM: Cleavage of precursor form by SENP1 or SENP2 is necessary for function. SIMILARITY: SwissProt: P63165 ## Belongs to the ubiquitin family. SUMO subfamily. & Contains 1 ubiquitin-like domain.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -80°C from date of shipment for up to 12 months. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80ºC.