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  • merck millipore,默克密理博,07-947,Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody

  • merck millipore,默克密理博,07-947,Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody

    产品名称：Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody
    产品型号：07-947
    Use Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody (rabbit polyclonal antibody) validated in WB, ICC, DB to detect dimethyl-Histone H4 (Arg3) Symmetric also known as histone H4, histone cluster 4  More>>

    merck millipore,默克密理博,07-947,Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody

  • 产品介绍
  • merck millipore,默克密理博,07-947,Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MICC, WBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 07-947
    描述 Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody
    Alternate Names
    • histone cluster 4, H4
    • histone 4, H4
    • histone H4
    背景信息 Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Arginine modifications of histones proteins are mediated by two classes of protein arginine methyltransferases (PRMTs), class I and II. Class I PRMTs place two methyl groups on the one nitrogen atom of the arginine guanidino group, resulting in an asymmetric modification. In contrast class II PRMTs place two methyl groups on two nitrogen atoms of the guanidino group producing a symmetric dimethylation. Although there are exceptions, asymmetric modifications are typically associated with transcription activation while symmetric modifications are most commonly associated with transcriptional repression.
    产品信息
    格式 Affinity Purified
    控制
    • HeLa acid extract
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Use Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody (rabbit polyclonal antibody) validated in WB, ICC, DB to detect dimethyl-Histone H4 (Arg3) Symmetric also known as histone H4, histone cluster 4 H4.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H4 in HeLa and A431 cells.

    Peptide Dot Blot Analysis: 0.5 µg/mL of this antibody detected Histone H4 dimethyl Arg3 (symmetric) peptides, but did not detect Histone H4 dimethyl Arg3 (assymmetric) peptide, other modified peptides, or non-methyated histone H4 peptides.
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to human Histone H4 when symmetrically dimethylated at Arg3.
    表位 Symmetrically dimethylated Arg3
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes Histone H4 symmetrically dimethylated at Arg3.
    品种反应性 RatHumanMouse
    Species Reactivity Note Demonstrated to react with human, mouse, and rat. Broad species cross-reactivity expected based on sequence similarity.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_001029249
    Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq].
    基因符号
    • H4/E
    • HIST1H4A
    • H4/J
    • HIST1H4F
    • HIST1H4L
    • H4FN
    • H4FA
    • H4FH
    • HIST1H4H
    • H4F2
    • H4FM
    • H4/A
    • H4FK
    • H4FG
    • HIST2H4
    • H4FB
    • H4/K
    • HIST1H4I
    • H4/N
    • HIST1H4B
    • H4FE
    • H4/M
    • H4/a
    • HIST1H4E
    • HIST4H4
    • HIST2H4A
    • H4FC
    • H4FI
    • H4/H
    • HIST1H4C
    • H4/G
    • HIST1H4K
    • H4FJ
    • H4FD
    • H4/I
    • H4/B
    • H4/D
    • H4/C
    • HIST1H4J
    • HIST1H4D
    修改
    • Methylation
    纯化方法 Affinity purified
    UniProt编号
    • P62805
    UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

    SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

    SUBCELLULAR LOCATION: Nucleus. Chromosome.

    PTM: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.

    Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.

    Monomethylation at Arg-4 (H4R3me1) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6.

    Symmetric dimethylation on Arg-4 (H4R3me2a) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.

    Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.

    Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).

    Sumoylated, which is associated with transcriptional repression.

    SEQUENCE SIMILARITIES: Belongs to the histone H4 family.

    SEQUENCE CAUTION: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3.
    产品使用声明
    质量保证 Evaluated by Western Blot in HeLa acid extract.

    Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H4 on 10 µg of HeLa acid extract.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,07-947,Anti-dimethyl-Histone H4 (Arg3) Symmetric Antibody

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