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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Serum Polyclonal Antibody 描述 产品目录编号 AB2962 Replaces AB1963 描述 Anti-N-WASP Antibody Alternate Names - neural Wiskott-Aldrich syndrome protein
- Wiskott-Aldrich syndrome gene-like
- Wiskott-Aldrich syndrome gene-like protein
- Wiskott-Aldrich syndrome-like
背景信息 Members of the Wiskott-Aldrich syndrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a ~63 kDa protein in hematopoietic cells, while N-WASP is observed as a ~65 kDa in many tissues, especially brain. 产品信息 格式 Serum 控制 - HEK293 cell lysates
演示 Rabbit serum containing 0.05% NaN3. 应用 应用 This Anti-N-WASP Antibody is validated for use in WB for the detection of N-WASP. 主要应用 - Western Blotting
生物信息 免疫原品种 Full-length human recombinant N-WASP protein. 宿主 Rabbit 特异性 The antibody recognizes human N-WASP. 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse, rat. Reactivity with other species has not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003932
Entrez基因汇总 The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes. [provided by RefSeq] 基因符号 - MGC48327
- N-WASP
- NWASP
UniProt编号 - P42768
UniProt汇总 FUNCTION: Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
SUBUNIT STRUCTURE: Binds to CDC42, RAC, NCK, FYN, SRC kinase FGR, BTK, ABL, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Binds the Arp2/3 complex.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton.
TISSUE SPECIFICTY: Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen. Ref.1
DOMIAN: The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
INVOLVEMENT IN DISEASE: Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
Defects in WAS are a cause of X-linked severe congenital neutropenia (XLN) [MIM:300299]. XLN is an X-linked immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
SEQUENCE SIMILARITIES: Contains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 1 WH2 domain.产品使用声明 质量保证 Evaluated on a representative lot by Western blot on HEK293 cell lysate using 1:1000 dilution of Anti-N-WASP. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C for up to 1 year from date of receipt. 包装信息 数量 100 µL
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