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品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-937 描述 Anti-HDAC7 Antibody Alternate Names - histone deacetylase 7A
- histone deacetylase 7
背景信息 Histone deacetylases (HDAC) are enzymes that regulates transcription by selectively deacetylating the eta-amino groups of lysines located near the amino termini of core histone proteins. Eight members of the HDAC family have been identified, divided in to two classes. Class I includes HDAC-1, 2, 3 and 8 while Class II includes HDAC-4, 5, 6, and 7. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a C-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional co-repressor complexes that can be recruited to specific promoter regions via interactions with transcription factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones, resulting in a tighter DNA-histone interaction. This compaction of chromatin leads to the repression of transcription of DNA in that region. 产品信息 格式 Affinity Purified 控制 - Recombinant HDAC7 protein (Cat. #14-832).
演示 Purified Rabbit polyclonal in buffer containing 20 mM PBS and 0.1% sodium azide. 应用 应用 Use Anti-HDAC7 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect HDAC7 also known as histone deacetylase 7, histone deacetylase 7A. 主要应用 - Western Blotting
应用说明 Western Blotting:
A previous lot of this antibody was shown to recognize recombinant HDAC7 but not recombinant HDAC1, HDAC4, or HDAC8.
Western Blotting:
A previous lot of this antibody was shown to recognize endogenous HDAC7 in HEK293T cells.
生物信息 免疫原品种 Synthetic peptide corresponding to amino acids 164-182 of human HDAC7 [(C)KPKKSLERRKNPLLRKESA]. 表位 a.a.164-182 宿主 Rabbit 特异性 This antibody recognizes human HDAC7. Does not cross-react with HDAC1, HDAC4, or HDAC8. 品种反应性 Human Species Reactivity Note Reacts with Human. Not tested with other species. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_001091886.1
Entrez基因汇总 Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq].
基因符号 - HDAC7A
- DKFZP586J0917
- EC 3.5.1.98
- FLJ99588
- HD7
- HD7A
- HD7a
纯化方法 Affinity Purfied UniProt编号 - Q8WUI4
UniProt汇总 FUNCTION: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors By similarity. May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
Catalytic activity Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.
SUBUNIT: Interacts with HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, NCOR1, NCOR2, SIN3A, SIN3B, RBBP4, RBBP7, MTA1L1, SAP30 and MBD3. Interacts with the 14-3-3 protein YWHAE, MEF2A, MEF2B and MEF2C By similarity. Interacts with HTATIP and EDNRA. Interacts with JARID1B.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note= In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
DOMAIN:The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm By similarity.
PTM: May be phosphorylated by CaMK1 By similarity.
MISCELLANEOUS: Its activity is inhibited by Trichostatin A (TSA), a known histone deacetylase inhibitor By similarity.
SIMILARITY: Belongs to the histone deacetylase family. Type 2 subfamily.
产品使用声明 质量保证 Routinely evaluated by Western blotting using recombinant HDAC7 protein. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µL
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