merck millipore,默克密理博,HTS050M,ChemiScreen™ H1 Membrane Preparation

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merck millipore,默克密理博,HTS050M,ChemiScreen™ H1 Membrane Preparation
产品名称：ChemiScreen™ H1 Membrane Preparation
产品型号：HTS050M
Human H1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

merck millipore,默克密理博,HTS050M,ChemiScreen™ H1 Membrane Preparation

产品介绍

merck millipore,默克密理博,HTS050M,ChemiScreen™ H1 Membrane Preparation

重要规格表

应用说明	品种	宿主细胞	蛋白靶
Radioligand binding assay, and GTPγS binding.	Human	Chem-3	H1

描述
产品目录编号	HTS050M
品牌系列	Chemicon®
商名	Chemicon ChemiScreen
描述	ChemiScreen™ H1 Membrane Preparation
概述	Full-length human HRH1 cDNA
背景信息	Histamine exerts its biological effects through a family of four GPCRs. Stimulation of the H1 histamine receptor causes smooth muscle contraction, induces vascular permeability, and stimulates catecholamine release from the adrenal medulla. H1 is also widely expressed in brain, and activation of H1 excites several types of neurons by blocking potassium conductance (Hill et al., 1997). Chemicon's H1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of H1 interactions and its ligands. The membrane preparations exhibit a Kd of 4.5 nM for [3H]-Pyrilamine. With 3 nM [³H]-Pyrilamine, 5 mg/well and 10 mg/well H1 Membrane Prep typically yield greater than 3 and 6-fold signal-to-background ratios, respectively.

产品信息
演示	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein were adjusted to 0.5 mg/mL in 1 mL packaging buffer, rapidly frozen, and stored at -80°C.

应用
应用	Human H1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
应用说明	Radioligand binding assay, and GTPγS binding.

生物信息
浓缩	2 mL, 0.5 mg/mL
品种	Human
品种反应性	Human
Entrez基因编号	NM_001098213.1 NM_000861.3 NM_001098211.1 NM_001098212.1
Entrez基因汇总	Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene was thought to be intronless until recently. The protein encoded by this gene is an integral membrane protein and belongs to the G protein-coupled receptor superfamily. It mediates the contraction of smooth muscles, the increase in capillary permeability due to contraction of terminal venules, the release of catecholamine from adrenal medulla, and neurotransmission in the central nervous system. Multiple alternatively spliced variants, encoding the same protein, have been identified.
基因符号	hisH1 HRH1 H1-R
GPCR类别	A
宿主细胞	Chem-3
蛋白靶	H1
靶子系列	Histamine
UniProt编号	P35367
UniProt汇总	FUNCTION: SwissProt: P35367 # In peripheral tissues, the H1 subclass of histamine receptors mediates the contraction of smooth muscles, increase in capillary permeability due to contraction of terminal venules, and catecholamine release from adrenal medulla, as well as mediating neurotransmission in the central nervous system. SIZE: 487 amino acids; 55784 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. PTM: Potential sites of phosphorylation in the third cytoplasmic loop may play an important role in regulating signal transduction through the receptor molecule. SIMILARITY: SwissProt: P35367 ## Belongs to the G-protein coupled receptor 1 family.
孵化条件	Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Tris, pH 7.4, 10 mM MgCl₂, 1 mM EDTA, filtered and stored at 4°C Radioligand: [³H] Pyrilamine (Perkin Elmer#:NET594250UC ) Wash Buffer: 50 mM Tris, pH 7.4 filtered and stored at 4°C. One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 3-fold signal:background with 3H-labeled Pyrilamine at 3 nM

产品使用声明

质量保证

Signal:background and specific binding values obtained in a competition binding assay with varying amounts of TP membrane prep:

	10 µg/well
Signal:Background	6.6
Specific Binding (cpm)	925

SPECIFICATIONS: 1 unit = 10 µg for most lots. Specific activity (units/µg) may vary between lots.
Bmax: 8.3 pmol/mg
Kd: 4.5 nM
Note: Lot number MM1357015 contains 2 mL at 2 mg/mL, for which 1 unit = 20 µg

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.