merck millipore,默克密理博,07-892,Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody

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merck millipore,默克密理博,07-892,Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody
产品名称：Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody
产品型号：07-892
This Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody is validated for use in WB, IC for the detection of phospho-Pyk2/CAKβ/FAK2 (Tyr402).

merck millipore,默克密理博,07-892,Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody

产品介绍

merck millipore,默克密理博,07-892,Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	ICC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-892
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody
Alternate Names	CADTK PTK2B Proline-rich tyrosine kinase 2 Focal adhesion kinase 2 FADK2 Cell adhesion kinase beta RAFTK Calcium-dependent tyrosine kinase

产品信息
格式	Affinity Purified
演示	100 μl of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

应用
应用	This Anti-phospho-Pyk2/CAKβ/FAK2 (Tyr402) Antibody is validated for use in WB, IC for the detection of phospho-Pyk2/CAKβ/FAK2 (Tyr402).
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Pyk2 (Tyr402) in RIPA lysate of Pyk2 transfected CEF cells

生物信息
免疫原品种	Peptide corresponding to amino acid region encompassing the human and rat phospho-Pyk2/CAKβ/FAK2 (Tyr402).
宿主	Rabbit
特异性	Pyk2 phosphorylated on tyrosine residue 402
同种型	IgG
品种反应性	RatHuman
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_173176.1 NM_004103.3 NM_173174.1 NM_173175.1
Entrez基因汇总	This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
基因符号	PKB PTK2B RAFTK FAK2 PYK2 FADK2 PTK CADTK CAKB
修改	Phosphorylation
纯化方法	Affinity Purfied
UniProt编号	Q14289
UniProt汇总	FUNCTION: SwissProt: Q14289 # Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation. SIZE: 1009 amino acids; 115875 Da SUBUNIT: Interacts with Crk-associated substrate (Cas), PTPNS1 and SH2D3C (By similarity). Interacts with nephrocystin, DDEF2, OPHN1L, SKAP2 and TGFB1I1. SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Note=Interaction with nephrocystin induces the membrane-association of the kinase. TISSUE SPECIFICITY: Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney. Also expressed in spleen and lymphocytes. PTM: Phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by PKC activation. Recruitment by nephrocystin to cell matrix adhesions initiates Tyr-402 phosphorylation. In monocytes, adherence to substrata is required for tyrosine phosphorylation and kinase activation. Angiotensin II, thapsigargin and L-alpha- lysophosphatidic acid (LPA) also induce autophosphorylation and increase kinase activity (By similarity). SIMILARITY: SwissProt: Q14289 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.