merck millipore,默克密理博,07-819,Anti-phospho-EGFR (Tyr1148) Antibody

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merck millipore,默克密理博,07-819,Anti-phospho-EGFR (Tyr1148) Antibody
产品名称：Anti-phospho-EGFR (Tyr1148) Antibody
产品型号：07-819
This Anti-phospho-EGFR (Tyr1148) Antibody is validated for use in WB for the detection of phospho-EGFR (Tyr1148).

merck millipore,默克密理博,07-819,Anti-phospho-EGFR (Tyr1148) Antibody

产品介绍

merck millipore,默克密理博,07-819,Anti-phospho-EGFR (Tyr1148) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-819
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-EGFR (Tyr1148) Antibody

产品信息
格式	Affinity Purified
演示	100 μl of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.

应用
应用	This Anti-phospho-EGFR (Tyr1148) Antibody is validated for use in WB for the detection of phospho-EGFR (Tyr1148).
主要应用	Western Blotting
应用说明	Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr1148) in RIPA lysates from EGF stimulated A431 cells.

生物信息
免疫原品种	Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr1148)
宿主	Rabbit
特异性	Recognizes phospho-EGFR (Tyr1148)
品种反应性	Human
Species Reactivity Note	Predicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_201284.1 NM_005228.3 NM_201282.1 NM_201283.1
基因符号	mENA EGFR ERBB1 ERBB
修改	Phosphorylation
纯化方法	Immunoaffinity purified
UniProt编号	P00533
UniProt汇总	FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist. SIZE: 1210 amino acids; 134277 Da SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers. PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980]. SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain. MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 2 years at -20°C from date of shipment. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.