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品种反应性 主要应用 宿主 格式 抗体类型 M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-500 Replaces 04-240 品牌系列 Upstate
商名 - Upstate
描述 Anti-p47-phox (mouse) Antibody Alternate Names - cyclin-dependent kinase inhibitor 1B (p27, Kip1)
- Cyclin-dependent kinase inhibitor p27
- cyclin-dependent kinase inhibitor 1B
- cyclin-dependent kinase inhibitor 1B (p27
背景信息 Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state. 产品信息 格式 Purified 控制 - RAW 264.7 cell lysate.
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. 应用 应用 This Anti-p47-phox (mouse) Antibody is validated for use in WB for the detection of p47-phox (mouse). 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated, synthetic peptides containing the first 15 amino acids of the N-terminal and the last 15 amino acids of the C-terminus of mouse p47-phox. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes mouse p47-phox, MW ~47 kDa. 同种型 IgG 品种反应性 Mouse Species Reactivity Note Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000256
Entrez基因汇总 The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq]. 基因符号 - CDKN4
- CDKN1B
- P27KIP1
- MEN1B
- MEN4
- KIP1
- p27Kip1
纯化方法 Protein A purfied UniProt编号 - Q09014
UniProt汇总 FUNCTION: SwissProt: P46527 # Involved in G1 arrest. May mediate TGF beta-induced G1 arrest. Binds to and inhibits complexes formed by cyclin E-CDK2, cyclin A-CDK2, and cyclin D1-CDK4. Interaction with nucleoporin NUP50 is required for nuclear import and for degradation of phosphorylated p27Kip1 after nuclear import (By similarity).
SIZE: 198 amino acids; 22073 Da
SUBUNIT: Interacts with NUP50 and with UHMK1. Interacts with COPS5 subunit of COP9 signalosome complex, leading to its subsequent degradation (By similarity). Interacts with SPDYA and is found in a complex with both SPDYA and CDK2.
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. Upon cell cycle progression, mostly cytoplasmic (By similarity).
TISSUE SPECIFICITY: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
DOMAIN: SwissProt: P46527 A peptide sequence containing only AA 28-79 retains substantial KIP1 cyclin A/CDK2 inhibitory activity.
PTM: Phosphorylation on Ser-10 leads to nuclear export to the cytoplasm and promotes cell cycle progression (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE: SwissProt: P46527 # Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.
SIMILARITY: Belongs to the CDI family.产品使用声明 质量保证 Evaluated by western blot in RIPA lysates from RAW 264.7 cells.
Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected p47-phox in RIPA lysates from RAW 264.7 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 100 µg
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