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merck millipore,默克密理博,05-1312,Anti-Ubiquityl-Histone H2B Antibody, clone 56
产品名称：Anti-Ubiquityl-Histone H2B Antibody, clone 56
产品型号：05-1312
Anti-Ubiquityl-Histone H2B Antibody, clone 56 is a Mouse Monoclonal Antibody for detection of Ubiquityl-Histone H2B also known as H2BUb, Histone H2B (ubiquityl), HIST1H2B & has been validated in WB.
merck millipore,默克密理博,05-1312,Anti-Ubiquityl-Histone H2B Antibody, clone 56
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ChIP, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1312 商名 - Upstate
描述 Anti-Ubiquityl-Histone H2B Antibody, clone 56 Alternate Names - H2BK119Ub
- H2BUb
- Histone H2B (ubiquityl)
- HIST1H2B
- H2BUbK119
背景信息 Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). A number of studies have implicated ubiquitinated H2B in heterochromatic gene silencing and in the control of gene expression of several yeast genes. 产品信息 格式 Purified 控制 - HeLa cell lysate.
演示 Purified from ascites by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide. 应用 应用 Anti-Ubiquityl-Histone H2B Antibody, clone 56 is a Mouse Monoclonal Antibody for detection of Ubiquityl-Histone H2B also known as H2BUb, Histone H2B (ubiquityl), HIST1H2B & has been validated in WB. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
生物信息 免疫原品种 Immunogen used was a branched peptide corresponding to the ubiquitination site on human H2B. 表位 Ubiquityl Lys120 克隆 clone 56 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes histone H2B when it is ubiquitinated at Lys120 but not H2B in its unmodified state, or ubiquitin unconjugated to H2B. Mr ~25 kDa. 同种型 IgG2aκ 品种反应性 RatHumanMouse Species Reactivity Note Based on sequence homology, broad species cross-reactivity is expected. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_066406.1
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. 修改 - Ubiquitylation
纯化方法 Protein G purfied UniProt编号 - P62807
UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Subcellular location: Nucleus.
Post-translational modification: Monoubiquitination of Lys-120 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
产品使用声明 质量保证 Routinely evaluated by Western Blot
Western Blot Analysis:
A 0.25 μg/mL dilution of a recent lot detected ubiquityl histone H2B in HeLa cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
包装信息 数量 100 µg
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