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  • merck millipore,默克密理博,07-452,Anti-dimethyl-Histone H3 (Lys27) Antibody

  • merck millipore,默克密理博,07-452,Anti-dimethyl-Histone H3 (Lys27) Antibody

    产品名称：Anti-dimethyl-Histone H3 (Lys27) Antibody
    产品型号：07-452
    Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl More>>

    merck millipore,默克密理博,07-452,Anti-dimethyl-Histone H3 (Lys27) Antibody

  • 产品介绍
  • merck millipore,默克密理博,07-452,Anti-dimethyl-Histone H3 (Lys27) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 07-452
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-dimethyl-Histone H3 (Lys27) Antibody
    概述 Anti-dimethyl-Histone H3 (Lys27)
    Alternate Names
    • Histone H3 (di methyl K27)
    • H3K27me2
    背景信息 Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
    产品信息
    格式 Affinity Purified
    控制
    • HeLa whole cell lysate, U2OS cell lysate
    演示 Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.
    应用
    应用 Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).
    主要应用
    • Western Blotting
    应用说明 Additional Referenced Research Applications:
    Dot Blot, Immunocytochemistry (cells)
    生物信息
    免疫原品种 KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Recognizes dimethylated histone H3 (Lys27).
    同种型 IgG
    品种反应性 Human
    Species Reactivity Note Broad species cross-reactivity expected, based on sequence homology
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_003493
    Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
    基因符号
    • MGC126886
    • HIST3H3
    • H3FT
    • H3/t
    • H3t
    • MGC126888
    • H3/g
    • H3.4
    • H3T
    修改
    • Methylation
    纯化方法 Peptide affinity purified
    UniProt编号
    • Q16695
    UniProt汇总 FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
    SIZE: 136 amino acids; 15508 Da
    SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
    SUBCELLULAR LOCATION: Nucleus.
    TISSUE SPECIFICITY: Expressed in testicular cells.
    DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
    PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
    SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
    产品使用声明
    质量保证 Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 2 years at 2-8°C from date of shipment.
    包装信息
    数量 200 µg

    merck millipore,默克密理博,07-452,Anti-dimethyl-Histone H3 (Lys27) Antibody

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