merck millipore,默克密理博,07-432,Anti-K+/Cl- Cotransporter (KCC2) Antibody

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merck millipore,默克密理博,07-432,Anti-K+/Cl- Cotransporter (KCC2) Antibody
产品名称：Anti-K+/Cl- Cotransporter (KCC2) Antibody
产品型号：07-432
This Anti-K+/Cl-Cotransporter (KCC2) Antibody is validated for use in IC, WB for the detection of K+/Cl-Cotransporter (KCC2).

merck millipore,默克密理博,07-432,Anti-K+/Cl- Cotransporter (KCC2) Antibody

产品介绍

merck millipore,默克密理博,07-432,Anti-K+/Cl- Cotransporter (KCC2) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Ca, R	WB, ICC	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-432
品牌系列	Upstate
商名	Upstate
描述	Anti-K+/Cl- Cotransporter (KCC2) Antibody
Alternate Names	solute carrier family 12 (potassium-chloride transporter), member 5 Electroneutral potassium-chloride cotransporter 2 Erythroid K-Cl cotransporter 2 Neuronal K-Cl cotransporter electroneural potassium-chloride cotransporter 2 solute carrier family 12 (potassium-chloride transporter)
背景信息	KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporters. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.

产品信息
格式	Purified
控制	Rat brain membrane preparation.
演示	Purified rabbit polyclonal IgG in buffer containing 0.014 M Phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20°C.

应用
应用	This Anti-K+/Cl-Cotransporter (KCC2) Antibody is validated for use in IC, WB for the detection of K+/Cl-Cotransporter (KCC2).
主要应用	Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry: This antibody has been reported by an independent laboratory to detect K+/Cl- Cotransporter (KCC2) in Madin Darby Canine Kidney (MDCK) cells at 1:200 dilution.

生物信息
免疫原品种	N-terminal His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140 kDa. An unknown band >200 kDa was also detected.
同种型	IgG
品种反应性	CanineRat
Species Reactivity Note	Rat and dog. Wide range of species cross-reactivity expected due to sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_065759
Entrez基因汇总	K-Cl cotransporters are proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The protein encoded by this gene is an integral membrane K-Cl cotransporter that can function in either a net efflux or influx pathway, depending on the chemical concentration gradients of potassium and chloride. The encoded protein can act as a homomultimer, or as a heteromultimer with other K-Cl cotransporters, to maintain chloride homeostasis in neurons.
基因符号	KCC2 SLC12A5 KIAA1176 hKCC2
纯化方法	Protein A purfied
UniProt编号	Q9H2X9
UniProt汇总	FUNCTION: SwissProt: Q9H2X9 # Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons. SIZE: 1139 amino acids; 126184 Da SUBUNIT: Homomultimer and heteromultimer with other K-Cl cotransporters (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Brain specific. Detected in neuronal cells. SIMILARITY:SwissProt: Q9H2X9 ## Belongs to the SLC12A transporter family. MISCELLANEOUS: Inhibited by furosemide and bumetanide.

产品使用声明
质量保证	Routinely evaluated by western blot in rat brain membrane preperations. Western Blot Analysis: 1.0 µg/mL of this lot detected K+/Cl- Cotransporter (KCC2) in rat brain membrane preparations.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.