merck millipore,默克密理愽
  • merck millipore,默克密理博,07-2162,Anti-PCNA Antibody

  • merck millipore,默克密理博,07-2162,Anti-PCNA Antibody

    产品名称：Anti-PCNA Antibody
    产品型号：07-2162
    Use Anti-PCNA Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PCNA also known as proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin.

    merck millipore,默克密理博,07-2162,Anti-PCNA Antibody

  • 产品介绍
  • merck millipore,默克密理博,07-2162,Anti-PCNA Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Chp, H, M, RICC, WBRbPurifiedPolyclonal Antibody
    描述
    产品目录编号 07-2162
    描述 Anti-PCNA Antibody
    Alternate Names
    • Cyclin
    • DNA polymerase delta auxiliary protein
    • cyclin
    • proliferating cell nuclear antigen
    背景信息 Proliferating cell nuclear antigen (PCNA) belongs to the DNA sliding clamp family and is comprised of three identical monomers forming a heterodimer. Due to its unique structure, PCNA is able to increase the processivity of DNA polymerase delta by using its outer domains to be involved in protein-protein interaction while still tethered to and freely sliding along the DNA. PCNA is a critical regulatory component in the onset of leading strand DNA replication and the termination of lagging strand synthesis. Studies have also indicated a role for PCNA in the recruitment and regulation of MMR pathway repair proteins. PCNA is also able to interact with a wide variety of cell cycle proteins and serves to coordinate several proteins participating in DNA processes, such as apoptosis.
    产品信息
    格式 Purified
    控制
    • A431 cell lysate
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Use Anti-PCNA Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PCNA also known as proliferating cell nuclear antigen, cyclin, DNA polymerase delta auxiliary protein, Cyclin.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 Western Blot Analysis: 1 µg/mL from a previous lot detected PCNA on 10 µg of NIH/3T3 and PC12 cell lysates.

    Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected PCNA in A431 cells.
    生物信息
    免疫原品种 GST-tagged recombinant protein corresponding to human PCNA.
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    品种反应性 ChimpanzeeHumanMouseRat
    Species Reactivity Note Demonstrated to react with human, mouse, and rat. Predicted to react with chimpanzee based on 100% sequence homology. Other homologies: Bovine, rabbit, canine, and panda (99% sequence homology).
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_002583
    Entrez基因汇总 The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq].
    基因符号
    • PCNA
    纯化方法 Protein A
    UniProt编号
    • P12004
    UniProt汇总 FUNCTION: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.

    SUBUNIT STRUCTURE: Homotrimer (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6, APEX2 and POLDIP2. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A (By similarity). Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex.

    SUBCELLULAR LOCATION: Nucleus.

    PTM: Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.

    Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.

    MISCELLANEOUS: Antibodies against PCNA are present in sera from patients with systemic lupus erythematosus.

    SEQUENCE SIMILARITIES: Belongs to the PCNA family.
    产品使用声明
    质量保证 Evaluated by Western Blot in A431 cell lysate.

    Western Blot Analysis: 1 µg/mL of this antibody detected PCNA on 10 µg of A431 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,07-2162,Anti-PCNA Antibody

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