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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-206 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-SHC (Tyr317) Antibody 产品信息 格式 Affinity Purified 演示 0.2M Tris-glycine, 150mM NaCl, 0.05% sodium azide and 5mg/ml BSA before the addition of glycerol to 30% 应用 应用 Detect phospho-SHC (Tyr317) using this Anti-phospho-SHC (Tyr317) Antibody validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 peptide (FDDPS[pY]VN-C) corresponding to amino acids 312-319 of human p52 SHC, containing a C-terminal cysteine. This motif is the Grb2 binding site. 宿主 Rabbit 特异性 phosphorylated SHC protein isoforms 同种型 IgG 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003029
基因符号 - p66
- SHC1
- FLJ26504
- SHC
- p66SHC
- SHCA
- p52SHC
修改 - Phosphorylation
纯化方法 Immunoaffinity chromatography UniProt编号 - P29353
UniProt汇总 FUNCTION: SwissProt: P29353 # Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity).
SIZE: 583 amino acids; 62852 Da
SUBUNIT: Interacts with the Trk receptors in a phosphotyrosine- dependent manner. Interacts with the NPXY motif of tyrosine- phosphorylated IGF1R and INSR in vitro via the PID domain. Once activated, binds to GRB2. Interacts with tyrosine-phosphorylated CD3T. Interacts with the N-terminal region of APS. Interacts with phosphorylated LRP1. Interacts with INPP5D/SHIP1 and INPPL1/SHIP2.
SUBCELLULAR LOCATION: Cytoplasm. & Isoform p46Shc: Mitochondrion matrix. Note=Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.
TISSUE SPECIFICITY: Widely expressed. Expressed in neural stem cells but absent in mature neurons.
DOMAIN: SwissProt: P29353 In response to a variety of growth factors, isoform p46Shc and isoform p52Shc bind to phosphorylated Trk receptors through their phosphotyrosine binding (PID) and/or SH2 domains. The PID and SH2 domains bind to specific phosphorylated tyrosine residues in the Asn-Pro-Xaa-Tyr(P) motif of the Trk receptors. Isoform p46Shc and isoform p52Shc are in turn phosphorylated on three tyrosine residues within the extended proline-rich domain. These phosphotyrosines act as docking site for GRB2 and thereby are involved in Ras activation (By similarity).
PTM: Phosphorylated by activated epidermal growth factor receptor. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-36 upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity).
SIMILARITY: Contains 1 PID domain. & Contains 1 SH2 domain.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from HFF cells stimulated with EGF 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -20°C 包装信息 数量 200 µL
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