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merck millipore,默克密理博,07-175,Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
产品名称：Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
产品型号：07-175
Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP.
merck millipore,默克密理博,07-175,Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Eq, M, H, R, Ca, Xn, Ch, B WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-175 描述 Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody Alternate Names - protein kinase JNK1
- JNK1 alpha protein kinase
- JNK1 beta protein kinase
- JUN N-terminal kinase
- Stress-activated protein kinase JNK1
- mitogen-activated protein kinase 8
- mitogen-activated protein kinase 8 isoform JNK1 alpha1
背景信息 JNK1(MAPK8) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-α) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrome c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. JNK1 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. The JNK pathway is critically involved in diabetes and levels are abnormally elevated in obesity. The cell-permeable JNK inhibitory peptide may have promise as a therapeutic agent for diabetes. 产品信息 格式 Affinity Purified 控制 - Untreated and Lambda phosphatase treated RAW264.7 cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Detect phospho-JNK (Thr183/Tyr185 using this Anti-phospho-JNK (Thr183/Tyr185, Thr221/Tyr223) Antibody validated for use in WB, EA & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 from RAW264.7 cell lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human JNK phosphorylated at Thr183/Tyr185 and Thr221/Tyr223. 表位 Phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes JNK when phosphorylated at Thr183/Tyr185 and Thr221/Tyr223 . 同种型 IgG 品种反应性 HorseMouseHumanRatCanineXenopusChickenBovine Species Reactivity Note Demonstrated to react with mouse and human. Predicted to react with rat, canine, xenopus, chicken, bovine, and equine based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_620634
Entrez基因汇总 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. 基因符号 - JNK1A2
- MAPK8
- JNK1
- JNK21B1/2
- PRKM8
- JNK-46
- JNK
- SAPK1
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - P45983
UniProt汇总 FUNCTION: JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta- 2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
COFACTOR: Magnesium.
SIZE: 427 amino acids; 48296 Da
SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP (By similarity).
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. ... hide » see more »产品使用声明 质量保证 Evaluated by Western Blot in untreated and lambda phosphatase treated RAW264.7 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected JNK on 10 µg of untreated and lambda phosphatase RAW264.7 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µg
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