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  • merck millipore,默克密理博,07-1594,Anti-Phospho-Dnmt1(Ser714) Antibody

  • merck millipore,默克密理博,07-1594,Anti-Phospho-Dnmt1(Ser714) Antibody

    产品名称：Anti-Phospho-Dnmt1(Ser714) Antibody
    产品型号：07-1594
    Anti-Phospho-Dnmt1(Ser714) Antibody is a rabbit polyclonal antibody for detection of Phospho-Dnmt1(Ser714) also known as CXXC finger protein 9, DNA (cytosine-5-)-methyltransferase 1 & DNA MTase HsaI h More>>

    merck millipore,默克密理博,07-1594,Anti-Phospho-Dnmt1(Ser714) Antibody

  • 产品介绍
  • merck millipore,默克密理博,07-1594,Anti-Phospho-Dnmt1(Ser714) Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HELISA, WBRbPurifiedPolyclonal Antibody
    描述
    产品目录编号 07-1594
    描述 Anti-Phospho-Dnmt1(Ser714) Antibody
    Alternate Names
    • DNA methyltransferase HsaI
    • CXXC finger protein 9
    • CXXC-type zinc finger protein 9
    • DNA (cytosine-5-)-methyltransferase 1
    • DNA MTase HsaI
    • DNA methyltransferase 1
    背景信息 Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown.
    产品信息
    格式 Purified
    控制
    • HeLa nuclear extract or EGF-treated whole HeLa cell extract.
    演示 Purified rabbit polyclonal in buffer containing 0.09% NaN3.
    应用
    应用 Anti-Phospho-Dnmt1(Ser714) Antibody is a rabbit polyclonal antibody for detection of Phospho-Dnmt1(Ser714) also known as CXXC finger protein 9, DNA (cytosine-5-)-methyltransferase 1 & DNA MTase HsaI has been validated in WB, ELISA, DB.
    主要应用
    • ELISA
    • Western Blotting
    应用说明 ELISA:
    This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).

    Dot blotting:
    This antibody has been shown by an outside laboratory to be specific for the phospho-Ser714 immunogen peptide, but not the unphosphorylated peptide, by dot blotting (1:500).
    生物信息
    免疫原品种 The immunogen was a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser714 of human Dnmt1.
    表位 Ser714
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody recognizes human Dnmt1 phosphorylated on Ser714.
    品种反应性 Human
    Species Reactivity Note Human. Not tested in other species.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_001370
    Entrez基因汇总 DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

    Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.
    基因符号
    • MGC104992
    • AIM
    • CXXC9
    • DNMT
    • Dnmt1
    • EC 2.1.1.37
    • FLJ16293
    • M.HsaI
    • MCMT
    修改
    • Phosphorylation
    纯化方法 Purified
    UniProt编号
    • P26358
    UniProt汇总 FUNCTION: Methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. Ref.17

    Catalytic activity S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

    SUBUNIT STRUCTURE: Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Component of complexes containing SUV39H1. Interacts with DNMT3A and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Ref.17 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13

    SUBCELLULAR LOCATION: Nucleus. Ref.13

    TISSUE SPECIFICITY: Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. Ref.12

    INDUCTION: Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle.

    SIMILARITY: Belongs to the C5-methyltransferase family.

    Contains 2 BAH domains.

    Contains 1 CXXC-type zinc finger.

    SEQUENCE CAUTION:The sequence AAD54507.1 differs from that shown. Reason: Erroneous gene model prediction.

    产品使用声明
    质量保证 Western Blot Analysis:
    1:250 dilution of this antibody detected Dnmt1 on 10 µg HeLa nuclear extract
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 400 µL

    merck millipore,默克密理博,07-1594,Anti-Phospho-Dnmt1(Ser714) Antibody

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