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merck millipore,默克密理博,07-1489,Anti-Phospho-JAK1 (TYR1022/1023) Antibody
产品名称：Anti-Phospho-JAK1 (TYR1022/1023) Antibody
产品型号：07-1489
Detect Phospho-JAK1 (TYR1022/1023) using this Anti-Phospho-JAK1 (TYR1022/1023) Antibody validated for use in WB.
merck millipore,默克密理博,07-1489,Anti-Phospho-JAK1 (TYR1022/1023) Antibody
- 产品介绍
Replacement Information Replacement Information 07-1489 is a recommended replacement for AB3850 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1489 Replaces AB3850 描述 Anti-Phospho-JAK1 (TYR1022/1023) Antibody Alternate Names - Janus Kinase 1
背景信息 JAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. They regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs (Signal Transducers and Activators of Transcription), which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal STAT transcription factors, which subsequently dimerize and translocate to the nucleus where they bind DNA. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor. Among a variety of sites, Tyrosines 1007 and 1008 (Tyr1007/1008) are sites of trans- or autophosphorylation in vivo and in vitro kinase reactions. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. JAK3 is required for signaling through cytokine receptors which share a common -chain. 产品信息 格式 Purified 控制 - A431
演示 Protein A and peptide affinity purified rabbit polyclonal IgG in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 µg/mL BSA, with 50% glycerol. 应用 应用 Detect Phospho-JAK1 (TYR1022/1023) using this Anti-Phospho-JAK1 (TYR1022/1023) Antibody validated for use in WB. 主要应用 - Western Blotting
应用说明 Optimal working dilutions must be determined by the end user. 生物信息 免疫原品种 KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Tyr1022/1023 of human Jak1. 表位 phospho-specific (Tyr1022/1023) 宿主 Rabbit 特异性 Recognizes Jak1 only when phosphorylated at tyrosine residues 1022/1023. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, rat and mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002227.2
Entrez基因汇总 Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. 基因符号 - EC 2.7.10.2
- JAK1
- JAK1B
- JAK-1
- JAK1A
修改 - Phosphorylation
纯化方法 Protein A and peptide affinity purified UniProt编号 - P23458
UniProt汇总 FUNCTION: SwissProt: P23458 # Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
SIZE: 1142 amino acids; 131957 Da
SUBUNIT: Interacts with IL31RA and SHB.
SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein. Note=Wholly intracellular, possibly membrane associated.
DOMAIN: SwissProt: P23458 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on pervanadate treated A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated JAK1 on 10 μg of EGF treated A431 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at –20ºC for 1 year from date of receipt, do not aliquot. Handling Recommendations: Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. 包装信息 数量 100 µL
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