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品种反应性 主要应用 宿主 格式 抗体类型 B WB, ELISA Rb Purified Polyclonal Antibody 描述 产品目录编号 AB2047 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Fibronectin Antibody 背景信息 Fibronectin (FN) is an extracellular adhesion molecule and it is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion.
Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. FN also can serve to organize cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound FN receptors on cell surfaces. The importance of fibronectin in cell migration events during embryogenesis has been documented in several contexts, e.g.: 1) mesodermal cell migration during gastrulation can be blocked by injection of Arg-Gly-Asp (RGD) tripeptides that block cellular FN receptors (integrins); 2) injection of anti-FN antibodies into chick embryos blocks migration of precardiac cells to the embryonic midline, and; 3) the patterns of FN deposition in developing vertebrate limbs determines the patterns of precartilage cell adhesion to the ECM, thereby specifying limb-specific patterns of chondrogenesis. {D. Marcey}.产品信息 格式 Purified 演示 Protein G affinity purified IgG fraction at 1.0 mg/mL in liquid PBS (0.01M phosphate, 0.09M NaCl) pH 7.2 with no preservatives. 应用 应用 Anti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA, IF, RIA, WB, IH(P). 主要应用 - Western Blotting
- ELISA
应用说明 Immunohistochemistry: 1:80 dilution for immunofluorescent staining of fresh frozen bovine skin and liver tissues. Acetone or methyl-carnoy fixation is also reactive.
Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 minutes), acetone or methyl-carnoy fixation; traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only.
Immunoblotting: 1:1000 of 2% deoxycholate + 10% SDS, 6M urea extracted bovine cell cultures (Kinsella, 2000). Antibody demonstates the appropriate twin bands at ~220kDa.
Radioimmunoassay
ELISA
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Fibronectin extracted and purified from bovine plasma. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Antibody reacts with bovine fibronectin, and demonstrates cross-reactivity of less than 0.1% with bovine collagens types I, III, IV by RIA at 1:10,000 dilution. 品种反应性 Bovine 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_212482.1
- NM_002026.2
- NM_054034.2
- NM_212474.1
- NM_212475.1
- NM_212476.1
- NM_212478.1
Entrez基因汇总 This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. 基因符号 - DKFZp686H0342
- DKFZp686I1370
- MSF
- FN
- CIG
- DKFZp686F10164
- LETS
- FINC
- FN1
- DKFZp686O13149
UniProt编号 - P02751
UniProt汇总 FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
SIZE: 2386 amino acids; 262607 Da
SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
PTM: Sulfated.
SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 100 µg
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