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Replacement Information Replacement Information 07-1487 is a recommended replacement for AB3806. 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Serum Polyclonal Antibody 描述 产品目录编号 07-1487 Replaces AB3806 描述 Anti-JAK3 Antibody Alternate Names - Tyrosine-protein kinase JAK3
- Janus Kinase 3
- Janus kinase 3 (a protein tyrosine kinase, leukocyte)
- Leukocyte janus kinase
- Leukocyte janus kinase
背景信息 JAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs, which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. JAK3 is required for signaling through cytokine receptors which share a common γ chain. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. The stress-activated protein kinase 1 (SAPK) family is also referred to as the jun N-terminal kinase family in light of the substrate preference of these serine/threonine kinases. In addition to regulating many biological processes including proliferation and apoptosis, the JNK/SAPK family has been implicated in many neurodegenerative diseases including Alzheimer disease, Parkinson's disease and Amyotrophic lateral sclerosis. 产品信息 格式 Serum 控制 - HeLa cell lysate
演示 Rabbit polyclonal IgG whole antiserum in buffer containing sodium azide. 应用 应用 Detect JAK3 using this Anti-JAK3 Antibody validated for use in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation: 5-10 uL of a previous lot of this antibody was used in immunoprecipitation.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide corresponding to amino acids 782-797 of mouse JAK3 (SDPTPGIPSPRDELC).
宿主 Rabbit 特异性 Recognizes JAK3 (Janus Kinase 3). 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Human and Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_000215.2
Entrez基因汇总 JAK3 encodes Janus kinase 3, a tyrosine kinase that belongs to the Janus family. JAK3 functions in signal transduction and interacts with members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations that abrogate Janus kinase 3 function cause an autosomal SCID (severe combined immunodeficiency disease). 基因符号 - EC 2.7.10.2
- JAK3
- JAKL
- LJAK
- JAK3_HUMAN
- L-JAK
- JAK-3
UniProt编号 - P52333
UniProt汇总 FUNCTION: SwissProt: P52333 # Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
SIZE: 1124 amino acids; 125099 Da
SUBUNIT: Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.
SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein (By similarity). Note=Wholly intracellular, possibly membrane associated (By similarity).
TISSUE SPECIFICITY: In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B- and M-forms are detected in cells both of hematopoietic and epithelial origins.
DOMAIN: SwissProt: P52333 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
PTM: Tyrosine phosphorylated in response to IL-2 and IL-4.
DISEASE: SwissProt: P52333 # Defects in JAK3 are a cause of autosomal recessive T-cell negative/B-cell positive type of severe combined immunodeficiency (SCID) [MIM:600802]; a condition characterized by the absence of circulating mature T-lymphocytes and NK cells, normal to elevated numbers of nonfunctional B-lymphocytes, and marked hypoplasia of lymphoid tissues.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain产品使用声明 质量保证 Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis:
1:500 dilution of this lot detected JAK3 on 10 μg of HeLa lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,407272,Integrin αvβ3 Antagonist, P11 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,06-501,Anti-STAT1 Antibody, CT
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