merck millipore,默克密理博,07-146,Anti-Histone H2A (acidic patch) Antibody

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merck millipore,默克密理博,07-146,Anti-Histone H2A (acidic patch) Antibody
产品名称：Anti-Histone H2A (acidic patch) Antibody
产品型号：07-146
Anti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB.

merck millipore,默克密理博,07-146,Anti-Histone H2A (acidic patch) Antibody

产品介绍

merck millipore,默克密理博,07-146,Anti-Histone H2A (acidic patch) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Yeast (S. cerevisiae), M, R, H, Ch	WB	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	07-146
品牌系列	Upstate
商名	Upstate
描述	Anti-Histone H2A (acidic patch) Antibody
Alternate Names	histone cluster 2, H2ac H2A histone family, member Q histone 2, H2ac histone IIa H2ac histone IIa
背景信息	Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.

产品信息
格式	Serum
控制	HeLa cell extract.
演示	Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide. Frozen solution.

应用
应用	Anti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB.
主要应用	Western Blotting
应用说明	Immunofluorescence: Recommend Anti-phospho-Histone H2A.X (Ser139), clone JBW301, catalog #05-636.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 88-97 (IRNDEELNKL-C) of human Histone H2A.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes Histone H2A, Mr 14 kDa.
同种型	IgG
品种反应性	Yeast (S. cerevisiae)MouseRatHumanChicken
Species Reactivity Note	Human, mouse, rat, yeast and chicken. Broad species cross-reactivity is expected.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_003508
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family.
基因符号	H2A HIST2H2AC H2A-GL101 H2AFQ H2A/r H2A/q MGC74460
纯化方法	Unpurified
UniProt编号	Q16777
UniProt汇总	FUNCTION: SwissProt: Q16777 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 129 amino acids; 13988 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: The chromatin-associated form is phosphorylated on Thr-121 during mitosis (Probable). & Deiminated on Arg-4 in granulocytes upon calcium entry. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. & Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1. & Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). SIMILARITY: SwissProt: Q16777 ## Belongs to the histone H2A family.

产品使用声明
质量保证	Routinely evaluated by western blot on acid extracts from HeLa cell lysate. Western Blot Analysis: 1:1000-1:5000 dilutions of this lot detected Histone H2A (acidic patch) in acid extracts from HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.