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merck millipore,默克密理博,07-1389,Anti-phospho-Smad3 (Ser423/425) Antibody
产品名称：Anti-phospho-Smad3 (Ser423/425) Antibody
产品型号：07-1389
Anti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP homolog 3 & has been validated in WB.
merck millipore,默克密理博,07-1389,Anti-phospho-Smad3 (Ser423/425) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 抗体类型 R, M, Mi, H WB Rb Polyclonal Antibody 描述 产品目录编号 07-1389 描述 Anti-phospho-Smad3 (Ser423/425) Antibody Alternate Names - mothers against decapentaplegic homolog 3
- MAD, mothers against decapentaplegic homolog 3
- MAD, mothers against decapentaplegic homolog 3 (Drosophila)
- Mothers against DPP homolog 3
- SMA- and MAD-related protein 3
- SMAD 3
- SMAD family member 3
- SMAD, mothers against DPP homolog 3
- SMAD, mothers against DPP homolog 3 (Drosophila)
- mad homolog JV15-2
- mad protein homolog
背景信息 Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression. 产品信息 演示 Unpurified rabbit serum in buffer containing 0.05% sodium azide. 应用 应用 Anti-phospho-Smad3 (Ser423/425) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-Smad3 (Ser423/425) also known as MAD Mothers against DPP homolog 3 & has been validated in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser423/425 of human Smad3. 表位 Phosphorylated Ser423/425 宿主 Rabbit 特异性 Detects phosphorylated Smad3 (Ser423 and Ser425). 品种反应性 RatMouseMinkHuman Species Reactivity Note Mouse and Mink. Predicted to react with Human and Rat based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_005902
基因符号 - hSMAD3
- DKFZp586N0721
- DKFZp686J10186
- HSPC193
- HsT17436
- JV15-2
- MADH3
- MGC60396
- Mad3
- hMAD-3
修改 - Phosphorylation
UniProt编号 - P84022
UniProt汇总 FUNCTION: Transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is a receptor-regulated SMAD (R-SMAD) By similarity.
SUBUNIT STRUCTURE: Interacts with HGS. Interacts with NEDD4L in response to TGF-beta. Interacts with TTRAP By similarity. Interacts with SARA (SMAD anchor for receptor activation); form trimers with another SMAD3 and the co-SMAD SMAD4. Interacts with JUN/FOS, vitamin D receptor, homeobox protein TGIF and TGIF2, PEBP2-alpha C subunit, CREB-binding protein (CBP), p300, SKI, SNON, ATF2, SMURF2, AIP1, DACH1 and TGFB1I1. Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3. Found in a complex with SMAD2 and TRIM33 upon addition of TGF-beta. Interacts with SMAD2 and TRIM33. Found in a complex with SMAD3, Ran and XPO4. Interacts with XPO4. Interacts with LBXCOR1 and CORL2.
SUBCELLULAR LOCATION: CytoplasmBy similarity. NucleusBy similarity. Note= In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 By similarity.
DOMAIN: The MH2 domain is sufficient to carry protein nuclear export.
PTM: Phosphorylated on serine by TGF-beta and activin type 1 receptor kinases.
INVOLVEMENT IN DISEASE: Defects in SMAD3 may be a cause of colorectal cancer (CRC) [MIM:114500].
SEQUENCE SIMILARITIES: Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
产品使用声明 质量保证 Evaluated by western blot on untreated and UV-treated AKR-2B cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect Smad3 in UV-treated AKR-2B cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
包装信息 数量 100 µL
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