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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1341 描述 Anti-PHLPP1 Antibody Alternate Names - SCN circadian oscillatory protein
- Pleckstrin homology domain-containing family E protein 1
- PH domain leucine-rich repeat protein phosphatase
- PH domain and leucine rich repeat protein phosphatase
- pleckstrin homology domain containing, family E (with<br />leucine rich repeats) member 1
- Suprachiasmatic nucleus circadian oscillatory protein
背景信息 PHLPP1 is the protein phosphatase responsible for dephosphorylation of the Akt (Ser473) site. Akt (Ser473) is the TORC2 (mTOR) phosphorylation activation site. 产品信息 格式 Affinity Purified 控制 - Human brain tissue lysate
演示 Purified rabbit serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide. 应用 应用 Anti-PHLPP1 Antibody detects level of PHLPP1 & has been published & validated for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the C-terminus of PHLPP1. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the C-terminal region of PHLPP1. 品种反应性 RatHumanMouse Species Reactivity Note Proven to react with Human, Mouse and Rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_919431
基因符号 - hSCOP
- EC 3.1.3.16
- KIAA0606
- MGC161555
- PHLPP1
- PLEKHE1
- SCOP
纯化方法 Affinity Purfied UniProt编号 - O60346
UniProt汇总 FUNCTION: Protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. May act as a negative regulator of K-Ras signaling in the membrane rafts.
CATALYTIC ACTIVITY: A phosphoprotein + H2O = a protein + phosphate.
COFACTOR: Binds 2 manganese ions per subunit By similarity.
ENZYME REGULATION: Insensitive to okadaic acid.
SUBUNIT STRUCTUE: Interacts with nucleotide free form K-Ras (KRAS) via its LRR repeats By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Nucleus.
TISSUE SPECIFICITY: Ubiquitous.
INVOLVEMENT IN DISEASE: Defects in PHLPP may be a cause of colon cancer. It is hifhly down-regulated in several colon cancer and glioblastoma cell lines with elevates AKT1 phosphorylation. Its reintroduction in such cell lines causes a strong suppression of tumor growth.
SEQUENCE SIMILARITIES: Contains 19 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain.
SEQUENCE CAUTION: The sequence AAH82244.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.
产品使用声明 质量保证 Evaluated by Western Blot in Human brain tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected PHLPP1 in Human brain tissue lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C for 1 year from date of receipt. 包装信息 数量 100 µg
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