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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1331 描述 Anti-phospho-Rictor (Ser1219) Antibody Alternate Names - hAVO3
- Rapamycin-insensitive companion of mTOR
- AVO3 homolog
背景信息 Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GµL. mTORC2 is activated by growth factors and other mitogenic signals. 产品信息 格式 Affinity Purified 控制 - Rictor protein immunoprecipitated from 293T cells transfected with either wildtype Rictor or mutant Rictor (S1219A)
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-phospho-Rictor (Ser1219) Antibody is an antibody against phospho-Rictor (Ser1219) for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human Rictor phosphorylated at Ser1219. 表位 Phosphorylated Ser1219 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes Rictor phosphorylated at Ser1219. 品种反应性 RatHuman Species Reactivity Note Demonstrated to react with Human. Predicted to react with Rat based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_689969
Entrez基因汇总 RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM]. 基因符号 - RICTOR
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - Q6R327
UniProt汇总 FUNCTION: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
SUBUNIT STRUCTURE: Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, LST8, PRR5, RICTOR and MAPKAP1. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to MTOR and PRR5 within the TORC2 complex. May interact with PRR5L.
PTM: Phosphorylated by MTOR; when part of mTORC2.
SEQUENCE SIMILARITIES: Belongs to the pianissimo family.
SEQUENCE CAUTION: The sequence CAH18135.1 differs from that shown. Reason: Wrong choice of CDS.产品使用声明 质量保证 Evaluated by Western Blot in Rictor protein immunoprecipitated from 293T cells transfected with either wildtype Rictor or mutant Rictor (S1219A).
Western Blot Analysis: 1.25 µg/mL of this antibody detected Rictor on 10 µg of Rictor protein immunoprecipitated from 293T cells transfected with either wildtype Rictor or mutant Rictor (S1219A).使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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