merck millipore,默克密理博,07-131,Anti-Sirt1(Sir2) Antibody

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merck millipore,默克密理博,07-131,Anti-Sirt1(Sir2) Antibody
产品名称：Anti-Sirt1(Sir2) Antibody
产品型号：07-131
Use Anti-Sirt1(Sir2) Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect Sirt1(Sir2) also known as Silencing information regulator 2-like, sirtuin 2.

merck millipore,默克密理博,07-131,Anti-Sirt1(Sir2) Antibody

产品介绍

merck millipore,默克密理博,07-131,Anti-Sirt1(Sir2) Antibody

Replacement Information
Replacement Information	07-131 is a recommended replacement for 05-707

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H, M	ChIP, ICC, WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-131
Replaces	05-707
品牌系列	Upstate
商名	Upstate
描述	Anti-Sirt1(Sir2) Antibody
Alternate Names	sirtuin type 2 SIR2-like protein 2 silencing information regulator 2-like silent information regulator 2 sir2-related protein type 2 sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2 sirtuin 2 homolog) 2 sirtuin 2
背景信息	Sirtuins are a family of closely related enzymes named after the yeast gene Sir2 (silent mating type information regulation 2). Members of the Sirtuin family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Sirtuins have been implicated in the regulation of aging, transcription, apoptosis and stress resistance. Sirt1 (also known as Sirtuin 1, Sir2L1, and Sir2a), the mammalian homolog of yeast Sir2, has been shown to deacetylate and thereby deactivate the p53 protein to regulate cellular processes.

产品信息
格式	Affinity Purified
控制	Jurkat cell lysate, human colon carcinoma or human lung squamous carcinoma.
演示	Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide and 30% gylcerol

应用
应用	Use Anti-Sirt1(Sir2) Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect Sirt1(Sir2) also known as Silencing information regulator 2-like, sirtuin 2.
主要应用	Chromatin Immunoprecipitation (ChIP) Immunocytochemistry Western Blotting
应用说明	Immunoprecipitation: 5 µL of a previous lot of this antibody has been shown to immunoprecipitate Sirt1 from 1 mg of a 3T3/A31 RIPA cell extract. Immunocytochemistry: This antibody has been reported to detect Sirt1 in 3T3 cells fixed with paraformaldehyde. Chromatin Immunoprecipitation: Published by Tiberi et al., (2012)

生物信息
免疫原品种	GST-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α.
表位	a.a. 1-131
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes Sirt1.
同种型	IgG
品种反应性	HumanMouse
Species Reactivity Note	Human and mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_019812
Entrez基因汇总	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two transcript variants result from alternative splicing of this gene.
基因符号	hSIRT1 SIR2L1 SIR2alpha hSIR2
纯化方法	Immunoaffinity chromatography
UniProt编号	Q923E4
UniProt汇总	FUNCTION: NAD-dependent deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Essential during embryogenesis and gametogenesis. Deacetylates 'Lys-382' of p53/TP53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD(+)/NADH, which is essential in skeletal muscle cell differentiation. Despite some ability to deacetylate histones in vitro, such activity is either weak or inexistent in vivo. CATALYTIC ACTIVITY: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. COFACTOR: Binds 1 zinc ion per subunit (By similarity). ENZYME REGULATION: Activated by resveratrol (3,5,4'-trihydroxy-trans-stilbene), butein (3,4,2',4'-tetrahydroxychalcone), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene), Isoliquiritigenin (4,2',4'-trihydroxychalcone), fisetin (3,7,3',4'-tetrahydroxyflavone) and quercetin (3,5,7,3',4'-pentahydroxyflavone) (By similarity). Inhibited by nicotinamide. SUBUNIT: Interacts with MLLT7/FOXO4, HES1, HEY2 and PML (By similarity). Found in a complex with PCAF and MYOD1. Interacts with p53/TP53. Interacts with TAF1B. SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Recruited to the nuclear bodies via its interaction with PML (By similarity). TISSUE SPECIFICITY: Widely expressed. Weakly expressed in liver and skeletal muscle. SIMILARITY: Belongs to the sirtuin family. SIMILARITY: Contains 1 deacetylase sirtuin-type domain.

产品使用声明
质量保证	Routinely evaluated by western blot on 3T3 nuclear extract. Western Blot Analysis: A 1:1000-1:5000 dilution of this lot detected Sirt1 in a 3T3 nuclear extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

1 year at -20°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.