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merck millipore,默克密理博,ABT286,Anti-methyl VCP/p97 Antibody (Lys315)
产品名称:Anti-methyl VCP/p97 Antibody (Lys315)
产品型号:ABT286
Detect Valosin-containing protein using this rabbit polyclonal antibody, Anti-methyl VCP/p97 Antibody (Lys315) validated for use in western blotting, IP & Immunofluorescence.
merck millipore,默克密理博,ABT286,Anti-methyl VCP/p97 Antibody (Lys315)
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABT286 描述 Anti-methyl VCP/p97 Antibody (Lys315) Alternate Names - VCP
- Transitional endoplasmic reticulum ATPase
- TER ATPase
- 15S Mg(2+)-ATPase p97 subunit
- Valosin-containing protein
背景信息 Valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. 产品信息 格式 Affinity Purified 控制 - HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 30% glycerol. 应用 应用 Detect Valosin-containing protein using this rabbit polyclonal antibody, Anti-methyl VCP/p97 Antibody (Lys315) validated for use in western blotting, IP & Immunofluorescence. 主要应用 - Western Blotting
应用说明 Western Blotting Analysis: 1 µg/mL from a representative lot detected methyl VCP/p97 (Lys315) in 10 µg of A431, C2C12, HEK293, HepG2, HUVEC, Jurkat, L6, mouse brain tissue lysate, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates.
Western Blotting Analysis: 2 µg/mL from a representative lot detected methyl VCP/p97 (Lys315) in VCP/p97 lysine methyltransferase wild type liver, kidney, brain, spleen, lung, and MEF cell lysates, and demonstrated a loss of signal in VCP/p97 lysine methyltransferase knock out liver, kidney, brain, spleen, lung, and MEF cell lysates (Prof. P. Falnes and Prof. A. Klugland, University of Oslo.).
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected methyl VCP/p97 (Lys315) in VCP/p97 methyltransferase wild type mouse embryo fibroblasts, and demonstrated a loss of signal in VCP/p97 methyl transferase knock out mouse embryo fibroblasts (Prof. P. Falnes and Prof. A. Klugland, University of Oslo.).生物信息 免疫原品种 Linear peptide corresponding to human VCP/p97 methylated at Lys315. 表位 Methylated Lys315 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes VCP/p97 methylated at Lys315. 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_009057
基因符号 - VCP
纯化方法 Affinity Purified UniProt编号 - P55072
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analyis: 1 µg/mL of this antibody detected methyl VCP/p97 (Lys315) in 10 µg HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.包装信息 数量 100 µg
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