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merck millipore,默克密理博,07-102,Anti-IGF2BP1/2 Antibody
产品名称：Anti-IGF2BP1/2 Antibody
产品型号：07-102
Anti-IGF2BP1/2 Antibody is a Rabbit Polyclonal Antibody for detection of IGF2BP1/2 also known as Hepatocellular carcinoma autoantigen p62, IGF2 mRNA-binding protein 2 & has been validated in WB & IP.
merck millipore,默克密理博,07-102,Anti-IGF2BP1/2 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-102 描述 Anti-IGF2BP1/2 Antibody Alternate Names - insulin-like growth factor 2 mRNA binding protein 2
- VICKZ family member 2
- IGF2 mRNA-binding protein 2
- IGF-II mRNA-binding protein 2
- Hepatocellular carcinoma autoantigen p62
背景信息 Insulin-like growth factor 2 mRNA-binding proteins are RNA-binding factors belonging to the IMP (IGF-II mRNA-binding protein) family of proteins. These proteins contain four K homology domains and two RNA recognition motifs. IGF2BP's have key roles in regulating the translation of target mRNAs. For the insulin-like growth factor 2 (IGF2) gene, IGF2BP's bind to the 5' UTR of the mRNA and regulate translation. IGF2BP's are expressed in a variety of fetal tissues such as; follicles in ovary, liver, lung, kidney, thymus, placenta. Expression has also been observed in the gonoocytes of testis, oocytes, semen, spermatagonia and in several cancers including; testicular, lung, kidney, trachea, and prostate. 产品信息 格式 Affinity Purified 控制 - HEK293 cell lysate
演示 Purified rabbit polyclonal containing PBS with 0.09% azide. 应用 应用 Anti-IGF2BP1/2 Antibody is a Rabbit Polyclonal Antibody for detection of IGF2BP1/2 also known as Hepatocellular carcinoma autoantigen p62, IGF2 mRNA-binding protein 2 & has been validated in WB & IP. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 4 µg/mL from a representative lot detected IGF2BP1/2 on 10 µg of HEK293 cell lysate.
Immunoprecipitation Analysis: 4 µg/mL from a representative lot was used by an independent laboratory in IP.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human IGF2BP1/2 at the C-terminus. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes IGF2BP1 and IGFBP2 at the C-terminus. 品种反应性 RatHuman Species Reactivity Note Demonstrated to react with human. Predicted to react with rat based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006537
Entrez基因汇总 This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. 基因符号 - IMP-1
- IGF2BP2
- IMP-2
- IMP2
- VICKZ2
- p62
- IGF2BP1
纯化方法 Affinity purified UniProt编号 - Q9NZI8
UniProt汇总 FUNCTION: RNA-binding factor that affects mRNA nuclear export, localization, stability and translation. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Regulates mRNA stability during the integrated cellular stress response (ISR) in stress granules (SGs). Stabilizes the BTRC/FBW1A mRNA from degradation by disrupting miRNA-dependent interaction with AGO2. Identified in a HCV IRES-mediated translation complex, that enhances translation at the Hepatitis C virus (HCV) RNA-replicon via the internal ribosome entry site (IRES), but does not affect 5'cap-dependent translation. Acts as a HIV-1 retrovirus restriction factor that reduces HIV-1 assembly by inhibiting viral RNA packaging, assembly and processing of HIV-1 GAG protein on cellular membranes. Binds to mRNAs in stress granules (SGs). Binds to the stem-loop IV of the 5'-UTR and to the variable region and the poly(U-C) motif of the 3'-UTR of the HCV RNA-replicon. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulates its subcellular localization and translation. Binds both to the coding region mRNA stability determinant (CRD) and to AU-rich sequences in the 3'-UTR of the MYC and CD44 mRNAs and stabilizes these mRNAs. Binds to the fourth and fifth exons of the oncofetal H19 and neuron-specific TAU mRNAs and regulates their localizations. Binds to the adenine-rich autoregulatory sequence (ARS) 5'-UTR of the PABPC1 mRNA and is involved in its translational repression. The RNA-binding activity to ARS is stimulated by PABPC1. Binds to the coding sequence region of BTRC/FBW1A mRNA and mediates stabilization of BTRC/FBW1A and MYC mRNAs in response to beta-catenin signaling. Binding to RNA employs a cooperative, sequential mechanism of homo- or heterodimerisation. Also involved in growth or survival of lung-cancer cells. Protects the MYC and MDR-1 mRNAs from cleavage by a endoribonuclease, thus prolonging their stabilities By similarity. Binds to the 3'-UTR axonal localization signal (ALS) of TAU mRNA (By similarity). Binds to a conserved 54-nucleotide element in the 3'-UTR of the beta actin mRNA known as the 'zipcode' (By similarity). Promotes translocation of the beta-actin mRNA to dendrites (By similarity). May act as a regulator of mRNA transport to activated synapses in response to synaptic activity (By similarity).
SUBUNIT STRUCTURE: Can form homo- and heterodimers with IGF2BP1 or IGF2BP3. Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Interacts (via the KH domains) with HIV-1 GAG (via the second zinc finger motif of NC). Associates (via the RRM domains and KH domains) with HIV-1 particles. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with DHX9, ELAVL2, HNRNPA2B1, HNRNPC, HNRNPH1, HNRNPU, IGF2BP2, IGF2BP3, ILF2, PABPC1 and YBX1. Found in a RNP granule complex with FMR1. Interacts with FMR1. Component of a multi subunit autoregulatory ribonucleoprotein complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1. Interacts through the third and fourth KH domains with PABPC1 in a RNA-independent manner. Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP By similarity. Interacts with ELAVL4 in a RNA-dependent manner By similarity. Associates with microtubules and polysomes (By similarity).
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Cell projection › lamellipodium. Cell projection › dendrite By similarity. Cell projection › dendritic spine By similarity. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Colocalized with H19 RNA at lamellipodia. Colocalized with CD44 mRNA in RNP granules. Nuclear export is mediated by XPO1/CRM1. In motile cells, is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). Present in the form of granules and into F-actin-rich protrusion of dendrites, spines and subsynaptic sites (By similarity). Colocalizes with beta-actin mRNA in dendrites and spines (By similarity). Exhibited rapid, bidirectional movements in dendrites and spines (By similarity). Neuronal depolarization by KCl induces its rapid efflux from the cell body into dendrites (By similarity).
TISSUE SPECIFICTY: Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary, gonocytes of testis, oocytes, spermatogonia and semen (at protein level). Expressed in testicular and lung cancer (at protein level). Expressed in kidney, prostate, trachea, testis and lung cancer.
INDUCTION: Up-regulated in response to beta-catenin activation. R
DOMAIN: The third and fourth KH domains encompass the protein dimerization motif and are necessary and sufficient for RNA binding. The four KH domains are important for granule formation and SGs targeting. Contains two nuclear export signals, situated within the second and fourth KH domains. The four KH domains are important to suppress HIV-1 infectivity.
PTM: Phosphorylated. Phosphorylation may influence mRNA translation (By similarity).
SEQUENCE SIMILARITIES: Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains.产品使用声明 质量保证 Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected IGF2BP1/2 on 10 µg of HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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