merck millipore,默克密理博,07-052,Anti-phospho-Phospholamban (Ser16) Antibody

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merck millipore,默克密理博,07-052,Anti-phospho-Phospholamban (Ser16) Antibody
产品名称：Anti-phospho-Phospholamban (Ser16) Antibody
产品型号：07-052
This Anti-phospho-Phospholamban (Ser16) Antibody is validated for use in WB for the detection of phospho-Phospholamban (Ser16).

merck millipore,默克密理博,07-052,Anti-phospho-Phospholamban (Ser16) Antibody

产品介绍

merck millipore,默克密理博,07-052,Anti-phospho-Phospholamban (Ser16) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Rb, M, R, H, B, Ca, Po	WB	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	07-052
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-Phospholamban (Ser16) Antibody
Alternate Names	cardiac phospholamban
背景信息	Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

产品信息
格式	Affinity Purified
控制	Bovine cardiac preparation, treated with PKA.
演示	Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C.

应用
应用	This Anti-phospho-Phospholamban (Ser16) Antibody is validated for use in WB for the detection of phospho-Phospholamban (Ser16).
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes phosphorylated phospho-lamban (Ser16), Mr 5-25 kDa. Additional non-specific bands may be detected, Mr 75 & 100 kDa, in some lysates.
同种型	IgG
品种反应性	RabbitMouseRatHumanBovineCaninePig
Species Reactivity Note	Bovine. Predicted to cross-react with human, mouse, rat, rabbit, pig and dog.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_002658
Entrez基因汇总	The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.
基因符号	phospholamban CMD1P OTTHUMP00000017092 PLB
修改	Phosphorylation
纯化方法	Immunoaffinity chromatography
UniProt编号	P26678
UniProt汇总	FUNCTION: SwissProt: P26678 # Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. SIZE: 52 amino acids; 6109 Da SUBUNIT: Homopentamer. SUBCELLULAR LOCATION: Membrane. TISSUE SPECIFICITY: Heart. PTM: Phosphorylated in response to beta-adrenergic stimulation. DISEASE:SwissProt: P26678 # Defects in PLN are the cause of dilated cardiomyopathy 1P (CMD1P) [MIM:609909]. Cardiomyopathy is a disorder characterized by cardiac dilation and reduced systolic function. SIMILARITY: SwissProt: P26678 ## Belongs to the phospholamban family.

产品使用声明
质量保证	Routinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA. Western Blot Analysis: 0.5-2 µg/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 µg PKA (Catalog # 14-114) for 10 minutes. DO NOT BOIL CARDIAC PREPARATION. Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by western blot on bovine cardiac preparation that was incubated with PKA.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10 µg PKA (Catalog # 14-114) for 10 minutes.
DO NOT BOIL CARDIAC PREPARATION.
Boiling may reduce the 25 kDa pentamer into tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.