merck millipore,默克密理博,07-038,Anti-Tip60 Antibody

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merck millipore,默克密理博,07-038,Anti-Tip60 Antibody
产品名称：Anti-Tip60 Antibody
产品型号：07-038
Use Anti-Tip60 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Tip60 also known as HIV-1 Tat interactive protein, K(lysine) acetyltransferase 5, K-acetyltransferase.

merck millipore,默克密理博,07-038,Anti-Tip60 Antibody

产品介绍

merck millipore,默克密理博,07-038,Anti-Tip60 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-038
品牌系列	Upstate
商名	Upstate
描述	Anti-Tip60 Antibody
Alternate Names	KAT5 60 kDa Tat interactive protein HIV-1 Tat interactive protein HIV-1 Tat interactive protein, 60kDa K(lysine) acetyltransferase 5 K-acetyltransferase Tat interacting protein, 60kDa cPLA(2)-interacting protein cPLA2 interacting protein Histone acetyltransferase KAT5
背景信息	Histone acetyltransferase HTATIP (Tip60). Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Direct binding of HTATIP to the TAT protein of the human immunodeficiency virus (HIV) might be an important feature for efficient TAT transactivation of HIV gene expression.

产品信息
格式	Purified
控制	Jurkat cell lysate and RIPA lysates
演示	Purified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20°C.

应用
应用	Use Anti-Tip60 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Tip60 also known as HIV-1 Tat interactive protein, K(lysine) acetyltransferase 5, K-acetyltransferase.
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 494-513 (RIDSKCLHF-TPKDWSKRGKW) of human Tip60.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes Tip60, Mr 60 kDa. Additional proteins were detected at 66 kDa and 80 kDa in some human and mouse cell lines.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human and mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_182710
Entrez基因汇总	The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants.
基因符号	cPLA2 EC 2.3.1.48 EC 2.3.1.- ESA1 HTATIP HTATIP1 PLIP TIP TIP60 Tip60
纯化方法	Protein A purfied
UniProt编号	Q92993
UniProt汇总	FUNCTION: SwissProt: Q92993 # Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to HTATIP polyubiquitination and targets it to degradation. SIZE: 513 amino acids; 58582 Da SUBUNIT: Interacts with the cytoplasmic tail of APP (By similarity). Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4. The NuA4 complex interacts with MYC and the adenovirus E1A protein. Interacts with ATM. Interacts with PHF17. Interacts with HIV-1 Tat. SUBCELLULAR LOCATION: Nucleus. Cytoplasm, perinuclear region. Note=Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region. PTM: Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity. & Ubiquitinated by MDM2, leading to its proteasome-dependent degradation. SIMILARITY: SwissProt: Q92993 ## Belongs to the MYST (SAS/MOZ) family.

产品使用声明
质量保证	Routinely evaluated by western blot on RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA. Western Blot Analysis: 0.5-2 μg/mL of this lot detected Tip60 in RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA.
销售限制	Due to license agreement restrictions, this product cannot be purchased for resale.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.