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merck millipore,默克密理博,06-923,Anti-Cdk1/Cdc2 (PSTAIR) Antibody
产品名称：Anti-Cdk1/Cdc2 (PSTAIR) Antibody
产品型号：06-923
Anti-Cdk1/Cdc2 (PSTAIR) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Cdk1/Cdc2 (PSTAIR) & has been validated in ICC, IP & WB.
merck millipore,默克密理博,06-923,Anti-Cdk1/Cdc2 (PSTAIR) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ca, Am, Ch, H, M, R, Sh, Dr, Mk WB, ICC Rb Purified Polyclonal Antibody 描述 产品目录编号 06-923 品牌系列 Upstate
商名 - Upstate
描述 Anti-Cdk1/Cdc2 (PSTAIR) Antibody 背景信息 Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for cdc2. 产品信息 格式 Purified 控制 - A431 cell lysate
演示 Purified rabbit serum in Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Cdk1/Cdc2 (PSTAIR) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Cdk1/Cdc2 (PSTAIR) & has been validated in ICC, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Fluorescent Immunocytochemistry Analysis:
A previous lot of this antibody was used in the confocal Immunofluorescent microscopy of A431 cells. Cells were grown, washed, fixed with formaldahyde, and permeabilized with NP40. The cells were triple stained with with Anti-Cdk1/Cdc2 (Red, Cy3), Phalloidin (Actin, Green, AlexaFluor®488), and DAPI (Blue, Nucleus). Positive nuclear staining.
Immunoprecipitation: 4 µg of a previous lot immunoprecipitated cdc2 from 500 µg of A431 RIPA lysate.
生物信息 免疫原品种 Synthetic peptide corresponding to residues 42-57 of human cdc2 kinase. The immunogen is identical in a wide range of species. 表位 N-terminus 宿主 Rabbit 特异性 Cdk1/cdc2 kinase 同种型 IgG 品种反应性 CanineAmphibianChickenHumanMouseRatSheepDrosophilaMonkey Species Reactivity Note Human, mouse and rat.
Predicted to react in drosophila, monkey, canine, sheep, chicken, frog and starfish. Wide species cross-reactivity is expected.抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001786
Entrez基因汇总 The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. 基因符号 - CDC28A
- CDC2
- CDK1
- MGC111195
- DKFZp686L20222
纯化方法 Antigen affinity purified UniProt编号 - P06493
UniProt汇总 FUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
SIZE: 297 amino acids; 34095 Da
SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by western blot on human A431, mouse 3T3 and/or rat L6 RIPA cell lysates.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected cdc2 from human A431, mouse 3T3 or rat L6 RIPA cell lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,ABT267,Anti-CELSR3 Antibody
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