merck millipore,默克密理愽
  • merck millipore,默克密理博,06-821-I,Anti-phospho-PKCepsilon Antibody (Ser729)

  • merck millipore,默克密理博,06-821-I,Anti-phospho-PKCepsilon Antibody (Ser729)

    产品名称：Anti-phospho-PKCepsilon Antibody (Ser729)
    产品型号：06-821-I
    Anti-phospho-PKCepsilon Antibody (Ser729) is a highly specific rabbit polyclonal antibody, that targets PKC epsilon & has been tested in western blotting.

    merck millipore,默克密理博,06-821-I,Anti-phospho-PKCepsilon Antibody (Ser729)

  • 产品介绍
  • merck millipore,默克密理博,06-821-I,Anti-phospho-PKCepsilon Antibody (Ser729)

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, H, RWBRbAffinity PurifiedPolyclonal Antibody
    描述
    产品目录编号 06-821-I
    描述 Anti-phospho-PKCepsilon Antibody (Ser729)
    Alternate Names
    • nPKC-epsilon
    • PKCepsilon
    • PKCe
    • Protein kinase C epsilon type
    背景信息 Protein Kinase C epsilon (PKC epsilon) is an ~87 kDa (migrates predominantly as 95 kDa in quiescient cells) member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC epsilon is an actin cytoskeleton regulating protein whose overexpression confers an oncogenic phenotype by affecting the ras signaling cascade. PKC epsilon is phosphorylated by PDK1 on threonine 566 and is then autophosphorylated on the turn motif threonine 710 and the hydrophobic motif serine 729. Catalytically inactive PKC epsilon is not phosphorylated on serine 729. Phosphorylation of serine 729 is mediated by mTOR-dependent pathways, plays an important role in regulating PKC epsilon subcellular localization, and is responsible for the appearance of the 92 kDa PKC epsilon species.
    产品信息
    格式 Affinity Purified
    演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Anti-phospho-PKCepsilon Antibody (Ser729) is a highly specific rabbit polyclonal antibody, that targets PKC epsilon & has been tested in western blotting.
    主要应用
    • Western Blotting
    应用说明 Western Blotting/Peptide Inhibition Analysis: A 1:1,000 dilution from a representative lot detected phospho PKCepsilon (Ser729) in 10 µg of NIH/3T3 cell lysate.
    生物信息
    免疫原品种 KLH-conjugated linear peptide corresponding to human PKC phosphorylated at Ser729.
    表位 Phosphorylated Ser729
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 This antibody demonstrates cross-reactivity against the PKCbeta (Ser661) isoform, PKCdelta (Ser655) isoform, and possibly PKCalpha (Ser729), all of which contain the GF(pS)Y motif.
    品种反应性 MouseHumanRat
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NP_005391
    基因符号
    • PKC3
    • PRKCE
    修改
    • Phosphorylation
    纯化方法 Affinity purified
    UniProt编号
    • Q02156
    产品使用声明
    质量保证 Evaluated by Western Blotting/Peptide Inhibition Analysis in C6 cell lysate.

    Western Blotting/Peptide Inhibition Analysis: A 1:1,000 dilution of this lot detected phospho PKCepsilon (Ser729) in 10 µg of C6 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8°C from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,06-821-I,Anti-phospho-PKCepsilon Antibody (Ser729)

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