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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB, ICC, IHC Rb Purified Polyclonal Antibody 描述 产品目录编号 06-536 Replaces 04-433 品牌系列 Upstate
商名 - Upstate
描述 Anti-Bak Antibody, NT Alternate Names - Bcl-2 homologous antagonist/killer
- Bcl-2-like 7 protein
- pro-apoptotic protein BAK
- Apoptosis regulator BAK
- BCL2-antagonist/killer 1
- BCL2-like 7 protein
背景信息 Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress. 产品信息 格式 Purified 控制 - Included Positive Antigen Control: Catalog # 12-301, non-stimulated human A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Purified rabbit polyclonal in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen liquid. 应用 应用 Detect Bak using this Anti-Bak Antibody, NT validated for use in IC, IH, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry (Paraffin):
5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate.生物信息 免疫原品种 Synthetic peptide (SEEQVAQQDTEEVFRSC) corresponding to amino acid residues 23-38 of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Specific for Bak, MW ~28 kDa. 同种型 IgG 品种反应性 MouseHumanRat Species Reactivity Note Human, mouse and rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001188
Entrez基因汇总 The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. 基因符号 - MGC117255
- BAK1
- BAK
- MGC3887
- BCL2L7
- BAK-LIKE
- OTTHUMP00000039620
- CDN1
纯化方法 Protein A purfied UniProt编号 - Q16611
UniProt汇总 FUNCTION: SwissProt: Q16611 # In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the a repressor Bcl-2 or its adenovirus homolog E1B 19k protein.
SIZE: 211 amino acids; 23409 Da
SUBUNIT: Forms heterodimers with Bcl-2, E1B 19k protein, and Bcl- X(L).
SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein (Potential).
TISSUE SPECIFICITY: Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
DOMAIN:SwissProt: Q16611 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
SIMILARITY: Belongs to the Bcl-2 family.产品使用声明 质量保证 Evaluated by Western Blot on HEK293 lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 200 µg
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