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品种反应性 主要应用 宿主 格式 抗体类型 H, B, R WB, ICC Rb Purified Polyclonal Antibody 描述 产品目录编号 06-520 品牌系列 Upstate
商名 - Upstate
描述 Anti-Na+/K+ ATPase α-1 Antibody 背景信息 Na+/K+ ATPase α-1 is a human gene and functions to maintain Na+ and K+ across the cell membrane which is important for osmoregulation. It is a component of (Na+/K+) ATPase, a transporter that catalyzes the hydrolysis of ATP and transports sodium and potassium ions across the plasma membrane. It also partners with Src and other signaling proteins. Binding of ouabain and other cardiotonic steroids to the (Na+/K+) ATPase activates Src, resulting in the assembly and activation of multiple signaling cascades. Binding of ouabain also induces a positive inotropic effect. Diseases associated with ATPase are Thyrotoxic periodic paralysis and hyperthyroidism. (References: Wikipedia) 产品信息 格式 Purified 控制 - Rat Brain Microsomal Prep. lysate.
演示 Purified antibody in buffer containing 0.1M Tris-Glycine (pH7.4) 150mM NaCl and 0.05% NaN3. 应用 应用 This Anti-Na+/K+ ATPase α-1 Antibody is validated for use in IC, IP, WB for the detection of Na+/K+ ATPase α-1. 主要应用 - Western Blotting
- Immunocytochemistry
生物信息 免疫原品种 GST-tagged ATPase. 表位 Cytoplasmic domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes the α-1 subunit isoform of Na+/K+ ATPase. 同种型 IgG 品种反应性 HumanBovineRat Species Reactivity Note Rat and Human. Expected to cross-react with Rat-100%, Mouse-99%; human, pig, rabbit, monkey-96%. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001001586
Entrez基因汇总 The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. 基因符号 - MGC51750
- ATP1A1
- G00-119-711
- Na
- +K
- MGC3285
纯化方法 Protein A purfied UniProt编号 - P05023
UniProt汇总 FUNCTION: SwissProt: P05023 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
SIZE: 1023 amino acids; 112896 Da
SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
PTM: Phosphorylation on Tyr-10 modulates pumping activity (By similarity).
SIMILARITY: SwissProt: P05023 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.产品使用声明 质量保证 Routinely evaluated by Immunoprecipitation on rat brain microsomal prep. lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
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