merck millipore,默克密理博,06-1289,Anti-Cyclin T1 Antibody

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,06-1289,Anti-Cyclin T1 Antibody
产品名称：Anti-Cyclin T1 Antibody
产品型号：06-1289
Use Anti-Cyclin T1 Antibody (Sheep Polyclonal Antibody) validated in WB to detect Cyclin T1 also known as CDK9-associated C-type protein, cyclin C-related protein.

merck millipore,默克密理博,06-1289,Anti-Cyclin T1 Antibody

产品介绍

merck millipore,默克密理博,06-1289,Anti-Cyclin T1 Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB	Sh	Serum	Polyclonal Antibody

描述
产品目录编号	06-1289
描述	Anti-Cyclin T1 Antibody
Alternate Names	subunit of positive elongation transcription factor b CDK9-associated C-type protein Human immunodeficiency virus-1 expression cyclin C-related protein cyclin T1 cyclin T1b human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
背景信息	Cyclin T1, which is also known as CCNT, is a member of the cyclin family and functions as a CDK kinase regulator. Cyclin T1 is one of regulatory cyclins (T1, T2a, T2b, and K) that is closely associated with CDK9 kinase forming a heterodimer called P-TEFb. This kinase complex is involved in phosporylating the carboxyl-terminal domain (CTD) of the large RNA polymerase II subunit which stimulate the elongation upon initiation. The cyclin T1/CDK9 complex has also been referred to as tat-associated kinase. Tat is an HIV protein that targets the cyclin T1/CDK9 complex to activate the transcription of the virus.

产品信息
格式	Serum
控制	HeLa cell lysate
演示	Sheep polyclonal serum with 0.05% sodium azide.

应用
应用	Use Anti-Cyclin T1 Antibody (Sheep Polyclonal Antibody) validated in WB to detect Cyclin T1 also known as CDK9-associated C-type protein, cyclin C-related protein.
主要应用	Western Blotting
应用说明	Western Blot Analysis: A 1:1000 dilution from a previous lot detected Cyclin T1 in 10 µg of HEK293, HeLa, HepG2, Jurkat, and PC3 cell lysates.

生物信息
免疫原品种	His-tagged recombinant protein corresponding to human Cyclin T1.
表位	Unknown
宿主	Sheep
特异性	This antibody recognizes Cyclin T1.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Proven to react with human and mouse. Rat (85% sequence homology).
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001231.2
Entrez基因汇总	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit. [provided by RefSeq]
基因符号	HIVE1 CCNT1 CCNT CYCT1 CycT1 Cyclin-T
纯化方法	Unpurified
UniProt编号	O60563
UniProt汇总	FUNCTION: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. SUBUNIT STRUCTURE: Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with MDFIC. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitously expressed. MISCELLANEOUS: Interaction between Tat and cyclin-T1 requires zinc. SEQUENCE SIMILARITIES: Belongs to the cyclin family. Cyclin C subfamily.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa cell lysate. Western Blot Analysis: A 1:1000 dilution of this antibody detected Cyclin T1 in 10 µg of HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

包装信息
数量	100 µL

merck millipore,默克密理博,06-1289,Anti-Cyclin T1 Antibody

上一件merck millipore产品：merck millipore,默克密理博,CBL118-K,HLA Class II Dq Ms X Hu
下一件merck millipore产品：merck millipore,默克密理博,ECM440,β Integrin Screening Kit

whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech