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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-644 品牌系列 Upstate
商名 - Upstate
描述 Anti-CAR Antibody, clone RmcB Alternate Names - coxsackie virus and adenovirus receptor
- 46 kD coxsackievirus and adenovirus receptor (CAR) protein
- CVB3 binding protein
- CVB3-binding protein
- Coxsackievirus B-adenovirus receptor
- coxsackie virus B receptor
背景信息 The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR's biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation. 产品信息 格式 Purified 控制 - Lung tissue
演示 Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4. 应用 应用 Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry:
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.生物信息 免疫原品种 Native human coxsackie virus-adenovirus receptor protein. 克隆 RmcB 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes CAR at MW ~46 kDa. 同种型 IgG1 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001338
Entrez基因汇总 The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. 基因符号 - HCAR
- CXADR
- CAR
- hCAR
- HCVADR
纯化方法 Protein G purfied UniProt编号 - P78310
UniProt汇总 FUNCTION: SwissProt: P78310 # Receptor for group B coxsackieviruses and subgroup C of adenoviruses (AD2 and AD5), susceptibility to infection is correlated to its level of expression and availability at the plasma membrane of cells.
SIZE: 365 amino acids; 40030 Da
SUBUNIT: Monomer. Probably homodimer formed by 2 molecules on adjacent cells. Interacts with LNX, MPDZ, BAIAP1, DLG4, PRKCABP, TJP1 and CTNNB1. Secreted isoform 3, isoform 4 and isoform 5 can interact with the extracellular domain of the receptor. Binds adenovirus 12 fiber protein. Isoform 5 but not isoform 3 binds to coxsackievirus.
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 2: Cell membrane; Single-pass type I membrane protein. Note=In epithelial cells localizes to the apical junction complex composed of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted.
TISSUE SPECIFICITY: Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitoulsy expressed while isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.
DOMAIN: SwissProt: P78310 The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML. & The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.
PTM: N-glycosylated (By similarity). & Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.
SIMILARITY: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.产品使用声明 质量保证 Evaluated by Western Blot on mouse small intestine lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 200 µg
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